熱和化學(xué)物質(zhì)最佳誘導(dǎo)枯草桿菌芽孢萌發(fā)條件的研究.pdf

1、獨(dú)創(chuàng)性聲明本人聲明所呈交的論文是我個(gè)人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果?！M我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含為獲得寧夏大學(xué)或其它教育機(jī)構(gòu)的學(xué)位或證書而使用過的材料。與我一同工作的同志對(duì)本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示了謝意。研究生簽名：立、電乞時(shí)間：如lb年S月26日時(shí)間：如lb年S月2[日關(guān)于論文使用授權(quán)的說明本人完全了解寧夏大學(xué)有關(guān)保留、

2、使用學(xué)位論文的規(guī)定，即：學(xué)校有權(quán)保留送交論文的復(fù)印件和磁盤，允許論文被查閱和借閱，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。同意寧夏大學(xué)可以用不同方公式在不同媒體上發(fā)表、傳播學(xué)位論文的全部或部分內(nèi)容。(保密的學(xué)位論文在解密后應(yīng)遵守此協(xié)議)研勝簽名：l贏缸獺為呼：；時(shí)間∥)oc()年j月26日f時(shí)間：)。l()’／年主冠)bFtAbstractSporesareextremelyresistanttovarioustreatm

3、ents，butgerminatedsporescouldbeinactivatedeasilyTherearemanyfactorsthatinducethegerminationofsporesThisarticlestudiedthegerminationeffectsofBacillussubtilissporesbytemperaturepretreatmentcombinedwithchemicalsThisarticlet

4、reatedthesporeswithdifferentchemicals，differentcombinationsofchemicals，temperaturepretreatmentcombinedwithchemicals，combinationsofgerminationinducersandinlaibitorsThegerminationrateWasusedastheresearchindexandthegerminat

5、ioneffectsofBacillussubtilissporestreatedwithvariousschemeswereresearchedsystemicallyThestudyresultsare勰follows：(1)Atfirstthisarticlestudiedgermination—inducingeffectsofchemicalsonsporesItWasfoundthatDPA，inosine，dodecyla

6、mine，LalanineandDglucosecouldquicklyinducesporegerminationOD600valueandrefractilityofsporesdecreasedquicklyAt37“C，thegerminationinducingeffectsofDPA，D—glucoseandinosinewerehigherthanthatof25。CHoweverat250C，thegermination

7、inducingeffectsofLalanineanddodecylaminewerehigherthanthatof37“C，andthegerminationefficiencyofDPAWassignificantlyhigherthanthatofotherchemicalsubstancesWiththeincreaseofprocessingtime，thegerminationrateofsporesduringthef

8、irst10minutesincreasedrapidlyAfter10minutes，ittendedtobestableandthechangeofgerminationrateWasnotsignificant(2)TheeffectsofthecombinationofthermalpretreatmentandchemicalsubstancesonthegerminationofsporeswerestudiedItwasf

9、oundthatafterpretreatmentat60。Cfor15min，thegerminationrateofsporestreatedwithDPAWasincreasedsignificantlyItsgerminationratecouldreachmorethan98％ThermalpretreatmentcouldincreasethegerminationrateofBacillussubtilissporestr

10、eatedwiththecombinationofLalanineanddodecylamine，andthesporesgerminationrateswithpretreatmentat40℃werehigherthanthatof60℃TMEresultsindicatedthattreatmentofDPAcombinedwithpretreatmentcouldfHrtherreducetherefractilityofthe

11、spores(3)ThesporegerminationWasinducedbyacombinationofdifferentsporegermination—inducingagentsItWasfoundthatcombinationofDPAanddodecylamine，andcombinationofDPAandLalaninecouldinducesporegerminationHoweverthegerminationra

12、tesofsporestreatedwiththesecombinationswerelowerthanthatofDPAaloneHoweverthegerminationinducingeffectsofsomecombinationsofchemicalswerenotsignificantlySomecombinationsofchemicalsevencouldnotinducesporegermination(4)Thisa

13、rticlestudiedthegerminationinhibitingeffectsofthecombinationofDalanine、HgCl2、NaHC03andgerminationinducersonsporesItwasfoundthatthegerminationinhibitingeffectsofHgCl2wasmuchhigherthanthatofDalanineandNaHC03，andtheinhibitn

14、geffectsofDalaninewerehigherthanthatofNanC03ThesporegerminationwasnotinhibitedwhenitWastreatedwiththecombinationofdodecylamineandNaHC03，andcombinationofLalanineandNaHC03Withtheincreaseoftreatmenttime，thegerminationinhibi