檸條錦雞兒遺傳多樣性研究.pdf

1、絮目蔗遺犬等博士學(xué)位論文檸條錦雞兒遺傳多樣性研究聊73ZIOStudyongeneticdiversityofCaraganakorshinskiikom研究生：王贊指導(dǎo)教師：合作指導(dǎo)教師：申請學(xué)位門類級別：專業(yè)名稱：研究方向：所在學(xué)院：韓建國教授高洪文研究員農(nóng)學(xué)博士草業(yè)科學(xué)牧草種子、牧草育種及生物技術(shù)動物科技學(xué)院二零零五年六月本論文受國家“863”現(xiàn)代農(nóng)業(yè)主題“抗旱耐鹽堿生態(tài)環(huán)境建設(shè)用林灌木檸條、沙棘等新品種選育”(2002AA241

2、091)資助AbstractBasedonthenaturaldistributionofCaraganakorshinxkiiKonkinChinatenpopulationsofCkorshinskii(PGHQl，HQ2fmmShanxiprovince；SM，YLJBDBfromShaenxiprovince；EIK郴，DSfromInnerMongolia)wereselectedandinvestigatedGeneticd

3、iversities、Ⅳmevaluatedandcomparedatthreelevelofmorphology(i—eleaves，podsandseedsetc)，allozymeandDNAMainresultsobtainedaIeasfollows：(1)Molphologicaldiversitiesamong／withinpopulationswemdiscussedbyanalyzingcharacterssuchas

4、leavespo如’andseedsAnalysisofvarianceforallcharacteristicsw&ssignificantly礎(chǔ)hemamongpopulationsandamongindividualswithinpopulationThemeanphenotypicdifferentiationcoefficient(Vsr=0195)showsthatthevariationamongpopulations(8

5、05％、issagnificanayhigherthanthatwithinpopulationsThecorrelationsbetweenthephenotypictraitsandecologicalandgeographicfactorsarenotsignificanL1000seedmass，podwidthandleaflengthwlglethemaintraimsaco)eotforthepheuotypicvaria

6、tionsaccoI【UngtothePCAanddiscimkmmmuaysi&BasedonthedusteranalysisandPeAthepopulationsofCkorshinskiiinvestigatedmaybedividedintofourgroupsbyphenotypictraits(2)Agelelectmphoresismethodwasusedtostudythegeneticdiversityoften

7、Ckorsh／ns蜮populationsEightof11locifromsevenenzymesassayedwerepolymorphiaCkorsh／nsk／／maintainedhighlevelofgeneticvariationascomparedwiththeaverageotherspeciesAtspecieslevelthenxannumberofallelesperlocus印)wasl727，themeanef

8、fectiveⅡu硼褂ofallelesperlocusa)wasl533，thepercentageof辨母妯。枇loci∞was727％，theebserveAk期∞蜀宮攄ityfito)吣0A25，the∞甲cctedlkmozygnsityQmw舔0292AtpopulationleveltheesliDulll強w訛』=1618A。群1470，P=618％，矗：=0408，and月：=0263The腑ngenedifferen

9、tiationcoefficient(G白)was014indicatingthatapproximately848％ofthetotalaBozymevariationoccurredwithinpopulationsThegeneflowamongpopulations帆)was15AccordingtotheUPGMAclusteranalysisbased∞thegeneticdistance，tenpopulationsofC

10、korgdns／d／wemdividedintothreegroupstherewasuosignificantrelationshipbetweengeneticdistanceandgeographicdistanceamongpopulations(3)ClearandhighresolutionDNAfingerprints、№mobtainedbySDSmethodTheoptimizedAFLPreactionsystemi

11、ncludeextractedgenomicD№《加0n砂q)wasdigestedwithEcoRlandMseIrestrictionenzymes；atotalvolumeof2Qulreactionmixture，thepreamplficationcousin2肛lofthedigesfon／Hgafionmixture，06蘆leachofthepreamplificationptimers(50ng／／M])，犁lIOxP

12、CRbuffer1犁IMg“(25raM)，016一ldNWl“Ps(25raM)01犁lTaqpolymeruse(5U／／d)；Selectiveamplificationreactionmixture(2陬1)waspreparedwith犁ldilutedpreamplificationproduction，08meachEcoRIandMselprimera岫咖1)，犁l10xPCRbuffer121alM礦(2SmM)018

13、蘆ldNTPs(25mM)o和lTaqpolymerase($U／p1)。Fourprimercombinationsptdiuced358bandsaczossatotalof98individualsHighgeneticdiversitywasbothobservedatspeA：icslevel(P=939％，日暑03168，I=0477)andpopulationslevel(Pj695％，H=0244，I=0365)Asig