表面增強拉曼散射的電磁場效應在生化分析中的應用.pdf

1、湖南大學碩士學位論文表面增強拉曼散射的電磁場效應在生化分析中的應用姓名：高星申請學位級別：碩士專業(yè)：分析化學指導教師：俞汝勤20080501表面增強拉曼散射的電磁場效應在生化分析中的應用IIIAbstractAccdingtoelectromagictheyofSERStheenhancementfactisproptionaltod12fasinglemoleculelocatedatdistancedfromthesurfaceof

2、substratealsotheaggregationofnanoparticlescouldgivestrongerSERSsignalsthanthoseisolatedparticles.Thepaperpresentedhereisfocusedonnanometalsurfaceenergytransfercocainedetectionbyusingaptamersynthesisofgoldaggregateddyeemb

3、eddedpolymerprotectednanoparticlesdescribedasfollows:1.Thevalidityoffluescentbehaviofrhodamine6GatdiscretedistancefromAg@Auceshellnanoparticlesurfacewhichfollowsad4distancedependencewasinvestigatedbySurfaceEnhancedRamanS

4、cattering(SERS).Theresultisincontradictiontotheliterature2.TheproofofprincipleofareagentlessaptamericsensbasedonsurfaceenhancedRamanscattering(SERS)spectroscopywith“signalon”architectureusingamodeltargetofcocainewasrepte

5、d.ThisnovelaptamericsenswasbasedontheconfmationalchangeofthesurfacetetheredaptameronbindingtargetthatdrawsacertainRamanrepterincloseproximitytotheSERSsubstratetherebyincreasingtheRamanscatteringsignalduetothelocalenhance

6、menteffectofSERS.Toimprovetheresponseperfmancethesensisfabricatedbyacocainetemplatedmixedselfassemblyof3’terminaltetramethylrhodamine(TMR)labeledDNAaptameronasilvercolloidfilmviaanalkahiolmoietyat5’end.Thisimmobilization

7、strategyoptimizestheientationofaptameronthesurfacefacilitatesthefoldingonbindingtarget.Underoptimizedassayconditionsonecandeterminecocaineataconcentrationof1Mwhichcomparesfavablywithanalogousaptamericsenssbasedonelectroc

8、hemicalfluescencetechniques.Thesenscanbereadilyregeneratedthroughwashingwithabuffer.TheseresultssuggestedthattheSERSbasedtrucermightcreateanewdimensionffuturedevelopmentofnovelaptamericsenssfsensitivedeterminationinbioch