微流體電泳芯片紫外-可見吸收檢測系統(tǒng)的構(gòu)建及應(yīng)用研究.pdf

1、中國科學(xué)院上海微系統(tǒng)與信息技術(shù)研究所碩士學(xué)位論文微流體電泳芯片紫外可見吸收檢測系統(tǒng)的構(gòu)建及應(yīng)用研究姓名：徐良基申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微電子學(xué)與固體電子學(xué)指導(dǎo)教師：徐元森趙建龍20040701StudyonMicro—FluidicElectrophoresisChipandUVAbsorptionDetectionSystemXuLiangji(Microelectronics)Directedby：XuYuansenZhaoJian

2、longABSTRACTMicro—fluidicElectrophoresisChip(MFEChip)isarapidlydevelopingfieldinrecentyearsItisamultidisciplinarytechnologyandderivedfromtheMicroElectroMechanicalSystem(MEMS)，biochemistryandanalyticalchemistryThemicro—ch

3、annelnetworkonthechipismanufacturedbytheMEMStechnologyonthesubstrateofglass，fusedsilica，PDMSetcSampleseparationisperformedinthemicro‘channelanddetectedbyOpticalorChemicalmethods，Ithasbeenappliedforlifescience，drugdiscove

4、ryandclinicmedicineInthisdissertation，bothelectrophoresisprincipleonMFEchipandthedesignanddevelopmentofMFEchipdetectionsystemarestudiedAsetofUVabsorptiondetectionsystemofMFEchiparealsoconstructedTheexperimentsresultsshow

5、edthatproteinsampleshavebeensuccessfullyseparatedanddetectedbyusingthissystem，Thetopicsofthisdissertationarepresentedbelow1ThemostrecentdevelopmentsofMFEchipsystemarereviewedandanoveldesignispresented；2，ThedesignofMFEchi

6、pislaidout，acompletesystemaremanufactured；3ThewholesystemisintegratedbysevenmodulesandoptimizedusingaUVabsorptionbaseddetectionsystem；4Thisdetectionsystemhasbeenusedforsomeproteinsamplesandurineseparationexperiments，ther

7、esultsarewellrepeatedandshowedthatithasmettheneedforproteinrapidseparationanddetectionThedissertationshowedthatMFEchiphassomeadvantagessuchashighspeedandlowsampleconsumingaswellaschip’sdesignflexibilityButhow的decreasethe