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1、<p><b>  畢業(yè)論文開題報(bào)告</b></p><p><b>  海洋生物資源與環(huán)境</b></p><p>  微型裸腹溞在不同餌料環(huán)境馴化下的攝食行為</p><p>  一、選題的背景與意義</p><p>  近幾十年隨著人類活動(dòng)的加劇,我國的淡水湖泊富營養(yǎng)化現(xiàn)象日趨嚴(yán)重。

2、湖泊富營養(yǎng)化常伴隨著夏季藍(lán)藻水華的爆發(fā)。微囊藻是藍(lán)藻水華的常見類群。因微囊藻缺乏營養(yǎng)價(jià)值并能產(chǎn)生微囊藻毒素(MC ) ,而被認(rèn)為是對(duì)浮游動(dòng)物有毒害作用的藻類。</p><p>  在淡水生態(tài)系統(tǒng)中,浮游動(dòng)物多以浮游植物為食,同時(shí)它們又是較高營養(yǎng)級(jí)生物的捕食對(duì)象,因此浮游動(dòng)物在淡水生態(tài)系統(tǒng)食物鏈中起著重要的樞紐作用。有研究表明水華藍(lán)藻對(duì)浮游動(dòng)物的危害不但會(huì)導(dǎo)致浮游動(dòng)物群落結(jié)構(gòu)的變動(dòng),而且對(duì)次級(jí)生產(chǎn)力也會(huì)產(chǎn)生深遠(yuǎn)的影

3、響,這些影響包括水華藍(lán)藻降低浮游動(dòng)物的存活率、抑制生長(zhǎng)和繁殖、降低攝食率等。</p><p>  枝角類可以攝食水華藍(lán)藻的代表——銅綠微囊藻。然而枝角類的品種不同,適應(yīng)溫度大小和生理狀況,微囊藻的存在狀態(tài)和數(shù)量,光照等都會(huì)枝角類攝食藍(lán)藻有影響,但這一方面的研究報(bào)道相對(duì)較少。本文希望通過研究銅綠微囊藻(Microcystis aeruginosa)對(duì)微型裸腹溞(Moina micrura)攝食行為的影響,確定經(jīng)自然

4、馴化和人工馴化后枝角類攝食行為是否改變,探討是否經(jīng)過馴化的微型裸腹溞比在對(duì)藍(lán)藻有更強(qiáng)的適應(yīng)性。從而為治理水華提供更好的理論依據(jù)。</p><p>  二、研究的基本內(nèi)容與擬解決的主要問題:</p><p>  研究基本內(nèi)容:在藍(lán)藻的自燃馴化條件下,藍(lán)藻對(duì)枝角類攝食行為的影響;</p><p>  在藍(lán)藻的人工馴化條件下,藍(lán)藻對(duì)枝角類攝食行為的影響。</p>

5、;<p><b>  擬解決的主要問題:</b></p><p>  如何借助別針和真空硅膠黏住枝角類的背部,并盡量降低對(duì)其活性的影響;</p><p>  如何于解剖鏡下觀察并準(zhǔn)確數(shù)出枝角類小顎的吞咽頻率和尾爪的拒食頻率。</p><p>  三、研究的方法與技術(shù)路線:</p><p><b>

6、  研究方法:</b></p><p>  在人工條件下馴化微型裸腹藻;</p><p>  獲取自燃馴化的微型裸腹藻;</p><p>  于解剖鏡下觀察并數(shù)出枝角類的小顎的吞咽頻率和尾爪的拒食頻率。</p><p><b>  技術(shù)路線: </b></p><p>  四、研究的總

7、體安排與進(jìn)度:</p><p>  1、20010.7-2010.9 微囊藻對(duì)微型裸腹藻攝食行為影響的實(shí)驗(yàn)、外文文獻(xiàn)翻譯;</p><p>  2、2010.11-2010.12 撰寫文獻(xiàn)綜述、開題報(bào)告、任務(wù)書等;</p><p>  3、2011.4-2011.5 數(shù)據(jù)整理,修改、完成論文定稿,畢業(yè)論文答辯。</p><p><

8、b>  五、主要參考文獻(xiàn):</b></p><p>  [1]蔣燮治,堵南山.中國動(dòng)物志節(jié)肢動(dòng)物門甲殼綱淡水枝角類.北京:科學(xué)出版社,1979. </p><p>  [2]鄭重,曹文清.海洋枝角類生物學(xué)[M].廈門:廈門大學(xué)出版社,1987.</p><p>  [3]Rohrlack T., Dittmann E., Henning M., B

9、orner T., Kohl J. G.., 1999. Role of microcystins in poisoning and food ingestion inhibition of Daphnia galeatacaused by the cyanobacterium Microcystis aeruginosa. Applied and Environmental Microbiology, 65: 737-7

10、39.</p><p>  [4]Thompson J. M., Ferguson A. J. D:, Reynolds C. S., 1982. Natural filtration rates of zooplankton in a closed system: the derivation of a community grazing index. Journal of Plankton Research,

11、 4(3): 545一560.</p><p>  [5] Peters R. H., 1984. Methods for the study of feeding, grazing and assimilation by zooplankton. In: Downing JA, Rigler FH, editors. A manual for the assessment of secondary produc

12、tion in fresh waters: IBP handbook 17, 2nd ed. Blackwell:Oxford, UK: 336-412.</p><p>  [6] Gliwicz Z. M.; Siedlar E., 1980. Food size limitation and algae interfering with food collection in Daphnia. Archiv

13、fiir Hydrobiologie, 88: 1 SS一177.</p><p>  [7] Porter K. G ., 1984. The energetic cost of response to blue-green algal filaments by cladocerans. Limnology and Oceanography, 29: 365-369.</p><p> 

14、 [8]Holm,N.P.,G.G. Gamf and J. Shapiro.Feeding and assimilation rates of Daphnia pulex fed aphyaraizomeraora flosaquae Limol. Oceangr . ,1983. 28:677- 687.</p><p>  [9] Demott, W. R. Feeding selectiveties an

15、d relative ingestion rates ofDaphniaandBosmina.Limnol. Oceanogr.,1982,27:516-527.</p><p>  [10] Fulton R S III, Paerl H W. Toxic and inhibitory effects of the blue-green alga Microcystis aeruginosa on herbiv

16、orous zooplankton[J]. Journal of Plankton Research, 1987, 9:837-855.</p><p>  [11] DeMott W R, Zhang Q X, Carmichael W W. Effects of toxic cyanobacteria and purified toxins on the survival and feeding of a c

17、opepod and three species of Daphnia[J]. Limnol Oceanogr, 1991, 36:1346-1357.</p><p>  [12] Reinikainen M, Walls M, Ketola M. Acute toxicity of the cyanobacterium Microcystis aeruginosa (strain PCC7820) to Da

18、phnia pulex (Cladocera)[J]. Algol Stud, 1994, 75:229-237.</p><p>  [13] Rohrlack T, Henning M, Kohl J G. Mechanisms of the inhibitory effect of the cyanobacterium Microcystis aeruginosa on Daphnia galeata’s

19、ingestion rate[J]. J Plankton Res, 1999, 21: 1489-1500.</p><p>  [14] Ferrão-Filho A S, Azevedo S, DeMott W R. Effects of toxic and non-toxic cyanobacteria on the life history of trophic and temperate c

20、ladocerans[J]. Freshwater Biol, 2000, 45: 1-19.</p><p>  [15] Chen F Z, Xie P. The effects of fresh and decomposed Microcystis aeruginosa on cladocerans from a subtropic Chinese lake[J]. J Freshwater Ecol, 2

21、003, 18: 97-104.</p><p>  [16]Carmichael W. W., 1992. Cyanobacteria secondary metabolites: the cyanotoxins. Jounal of Applied Bacteriology, 72: 445-459.</p><p>  [17] Mackintosh C., Beattie K. A

22、., Klumpp S., Cohen P., Codd G. A., 1990. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and hi啟her plants. FEBS Letters, 264: 187-192.</p><

23、;p>  [18] Codd G. A., Ward C. J., Bell S.G.,1997. Cyanobacterial toxins: occurrence, modes of action, health effects and exposure routes. Archives of Toxicology, 19: 3 99-410.</p><p>  [19] Jungmann D.,

24、Benndorf J., 1994. Toxicity to Daphnia of a compound extracted from laboratory and natural Microcystis spp., and the role of microcystins. Freshwater Biology, 32: 13-20.</p><p>  [20] Trabeau M, Bruha-Keup R

25、, McDerMott C, et al. Midsummer decline of a Daphnia population attributed in part to cyanobacterial capsule production[J]. Journal of Plankton Research, 2004, 26:949-961.</p><p>  [21] Fulton R S III, Paerl

26、 H W. Toxic and inhibitory effects of the blue-green alga Microcystis aeruginosa on herbivorous zooplankton[J]. Journal of Plankton Research, 1987, 9:837-855.</p><p>  [22] Jarvis A C, Hart R C, Combrink S.

27、Zooplankton feeding on size fractionated Microcystis colonies and Chlorella in a hypertrophic lake (Hartbeespoort Dam, South Africa): Implications to resource utilization and zooplankton succession[J]. Journal of Plankto

28、n Research, 1987,9(6): 1231-1249.</p><p>  [23] Romanovsky Y E., 1985. Food limitation and life-history strategies in Cladoceran crustaceans. Archiv fair Hydrobiologie, 21:363-372.</p><p>  [24]

29、 Ferrao-filho A. S., Azevedo S. M. F. O., Demott W. R., 2000: Effects of toxic and non-toxic cyanobacteria on the life history of tropical and temperature cladocerans. Freshwater Biology, 45: 1一19.</p><p&

30、gt;  [25] Hietala J, Laurén-Maatta C, Walls M. Sensitivity of Daphnia to toxic cyanobacteria: effects of genotype and temperature[J]. Freshwater Biol, 1997, 37:299-306.</p><p>  [26]何振榮, 俞家祿, 何家菀等. 東湖藍(lán)藻

31、水華毒性的研究II.季節(jié)變化及微囊藻的毒性[J]. 水生生物學(xué)報(bào),1989,20 (2): 192-194.</p><p>  [27] [20] Nandini S, Rao T R. Somatic and population growth in selected cladoceran rotifer species offered the cyanobacterium Microcystis aerug

32、inosa as food[J]. Aquatic Ecol, 1998, 31: 283-298.</p><p>  [28] Hanazato T. Interrelations between Microcystis and cladocera in the highly eutrophic Lake Kasumigaura, Japan[J]. Int Rev ges Hydrobiol, 1991,

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