溫郁金藥渣化學(xué)成分研究及栽培白術(shù)種質(zhì)遺傳多樣性分析.pdf

1、溫州醫(yī)學(xué)院碩士學(xué)位論文溫郁金藥渣化學(xué)成分研究及栽培白術(shù)種質(zhì)遺傳多樣性分析姓名：馬喜彥申請學(xué)位級別：碩士專業(yè)：中藥學(xué)指導(dǎo)教師：董建勇20100501溫州醫(yī)學(xué)院碩士學(xué)位論文StudiesontheConstistuentsofDregsfromCurcumawenyujinandAnalysisofGeneticDiversitiesofCulturedAtractylodesmacrocephalaABSTRACT‘≮Thethesisi

2、ncludestwoparts：thefirstpartiSaboutstudiesontheconstistuentsofdregsfromCurcumawenyujinYHChenetCLing；thesecondpartisaboutanalysisofgeneticdiversitiesofculturedAtratylodesmacrocephalaInthisthesis，thechemicalconstituentsoft

3、hedregsfromCurcumawenyujinYHChenetCLingwerestudiedOnthebasisofdifferentkindsofmordenchromatographyandidentificationmethods，8compoundsweredetermined，theyweresesquipenestheirstructureswereidentifiedascurcolide(1)，curdione(

4、2)，aerugidiol(3)，neocurdione(4)，alismoxide(5)，procurcumenol(6)，zedoalactoneI(7)，gajutsulactoneD(8)Inconclusion，thisreasarchhasprovidedrationalandexperimentialbasisforthecomplexutilizationofthisdregsofCurcumawenyujinYHChe

5、netCLingAtractylodesmacrocephalaisamemberofAtractylodesmacroephalaKoidz，whichisdistributioninZhejiangProvinceThecultivatedAtractylodesmacrocephalawerecollectedfromsixareasofTiantai，Xinchang，Panan，Dongyang，Shengzhou，Xianj

6、uinZhejiangProvincebytheprinciplesofuniformdistributionandrandomsampling6individualswerecollectedforeachtwonand36individualsintotalThecollectionregionsshouldCOVertheproductionareasaswidlyaspossibleAFLPwerecarriedouttoana

7、lyzethegeneticdiversityofcultivatedAtractylodesmacrocephalainthesesixpopulationsThepocessofAFLPisasfollows：RestrictionoftheDNAandligationofoligonucieotidcadapters；Selectiveamplificationofsetsofrestrictionfragments；Gelana

8、lysisoftheamplifiedfragments12606bandsweregeneratedby7pairsofAFLPprimercombinations，ofwhich10044werepolymorphic，thepercentageofpolymorphicWas7969％ThevarietyrangeofPPB％amongdifferentpopulationsis5417％5926％，5671％inaverage，

9、iSbetween02426“02646，02600inaverageNeiSbetween12549““12782，01648inavrageGstis00970，Nmis46547Thereisarelativehi曲geneticdiversitieslevelinZhejiangProvinceculturedAtractylodesmacrocephalaGeneticdifferentiationexistsamongpop