玉米轉(zhuǎn)錄因子基因cl-I-2 K1和r-2K1的克隆及其對轉(zhuǎn)基因煙草花色形成的影響.pdf

1、首都師范大學(xué)碩士學(xué)位論文玉米轉(zhuǎn)錄因子基因clI2K1和r2K1的克隆及其對轉(zhuǎn)基因煙草花色形成的影響姓名：王希慶申請學(xué)位級別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：印莉萍林忠平2003.6.1——亙疊墮蔓盔蘭堡主堂垡絲苧ABSTRACTAnthocyaninisonekindoftheflavonoid，anditisalsoanimportantplantsecondarvmetaboliteItcannotonlyaffecttheplantpo

2、lymorphism(especiallythecoloroftheflowers)，butalsothemetabolicprocessinplant(especiallytheprocessofreproduction)Thegenesencodingenzymesthatareneededduringtheanthocyaninsynthesisareconservativeinmanyhigherfloweringplants，

3、especiallyamongplantsthathavenearrelativeItimpliesthatthedifferenceamongtheregulativegenesmayaffectthecoloroftheflowersC1andRaretwokindsoftranscri【ptionfactorsfrommaizeC1isoneofaMYBtranscriptionalfactors，andR，belongstobH

4、LHfamilyTheyCaninteract謝theachotherandhaveimportantfunctionsduringanthocyaninsynthesisThen，cl一厶aIlalleleofcl，wasa]soisolatedfrommaizeItwasthoughttoactasatranscriptionalrepressorintheanthocyaninpathwayInourexperiment，wecl

5、onedtwogenesfrommaizeline‘‘2K1purple”，namelycl一12K1and，一2K1，andtheproteinstheyencodebelongtoMYBandbHLHproteinsrespectivelyThreebinaryexpressionvectorswereconstructed，whichharboredthecl—I2K1，，2K1，andc&&2K1r一2K1respective鞏

6、drivenbytheCaMV35SpromoterThen，Agrobacteriummediatedtransformationoftobacco(Nicotianatabacum，W38)wascarriedoutTransformedtobaccolineswereobtainedthroughtissuecultureandconfirmedbyPCRandsouthernblottinganalysisTheresultsi

7、ndicatethetobacco1inestransformedwitll吐／2K1havewhiteflowersandthosetransformedwithr2K1andc112K1一卜2j訂don’thaveobviouschangessimilartothatofthewildtobaccoSowecanspeculatethat：first，c&&2K1callrestrainthesynthesisofthetobacc

8、oanthocyanin；secondtheregulatorymechanismoftheanthocyaninsynthesismaybeconservativeamonghigherfloweringplants；third，therearenotobviouschangesintheflowersoftobaccolinestransformedwithr2K1；fourth，R一2K1proteinCaninhibitthee