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1、中國(guó)農(nóng)業(yè)科學(xué)院碩士學(xué)位論文基于TRFLP技術(shù)的肉雞消化道微生物群落多樣性的研究姓名:張華申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):動(dòng)物營(yíng)養(yǎng)與飲料科學(xué)指導(dǎo)教師:蔡輝益201106AbstractInthisdissertationmicrobialdiversityanditsd)r11amicchangesinbroilercaecumwerestudiedusingtechniqueofterminalrestrictionfragmentlength

2、polymorphism(TRFLP),andinfluencesofdietarychlortetracyclineandcellulose011growthofmicrobialcommunityinterminalileumofbroilerwerefurtherinvestigatedExperiment1TRFLPmethodonmicrobialcommunitydiversityanalysisinbroilergastr

3、ointestinaltractTRFLPmethodofstudyingthediversityofbroilergastrointestinalmicrobialcommunitywasestablishedbyoptimizingPCRconditions(selectionofprimeramountoftemplate,thereactionsystemannealingtemperature,andothers)accord

4、ingtocharacteristicsofmicrobial16SrDNAinbroilergastrointestinaltractRestrictionenzymedigestiontimewasalsoexploredPeal【ScannersoftwareandTRELPnetworkdatabasewereappliedinthemethodExperiment2Studyofdiversityanddynamicchang

5、姻ofmicrobialcommunityinbroilercecaTRFIPtechniqueWasusedinthistrialtostudythediversityanddyIlamicchangesofmicrobialcommunityinbroilercecatoexplorethemechanismhownutritionrelatedtogrowthanddevelopmentofgastrointestinalmicr

6、obialcommunityTheresultsshowedthatthececalmicrobialcommunitydiversityWasdifferentatdifferentages,butthedifferencewasnotsignificant(PO05)Asbirdsgrewtherichnessofcecalmicrobialcommunityspeciesdecreased,andthedominantspecie

7、sbecamemoreobviousIntherelativecontentofrepresentativeTRFsismorethan05%oftotaltheflorasofTRF36,Tl訌B7,TItF39,TRF57,TRFl64,TRFl68,TRFl88werenormalmicroflora,whiletheflorasofTRF52。TRFl86,TR脒weretemporaryfloraTI礎(chǔ)techniqueapp

8、liedinthistrialisagoodtooltoanalyzethediversityanddyIlamicchangesofmicrobialcommunityinbroilergutExperiment3EffectsofchlortetracyclineonmicrobialcommunitydiversityinbroilerterminalileumThistrialwasconductedtostudytheeffe

9、ctsofchlortetracyclineonmicrobialcommunitydiversityinbroilerterminalileumduringthestarterperiodaswellastoexplorethemicrobialdetectionmethodofchlortetracyclineTheresultsshowedthatduringthestarterperiodofbroilertheeffectso

10、fchlortetracyclineonmicrobialcommunitydiversityintheterminalileumwereexistManyTRFswereobtainedinthisexperimentandtherewere17TRFswhichwererepresentativeandtheirrelativecontentismorethan05%oftotalOftheseTI己Fs,TRF28。T砌P2l6。

11、TI己F596wereinhibited,whileTRF48,T剮昭7,TRF88,TRF95,TRFI68,TRF255werepromotedTheinfluenceofehlortetracyclineonunculturedmicroorganismsintheterminalileumcallbedetectedMoreimportantlythemicroorganisms(TRF596andTRF95)thatweres

12、ensitivetochlortetra_cyclineCanbedetectedaswellItCanbeconcludedthattwoTRFsmaybetheobjectivefragmentusedinthemicrobialdetectionofchlortetracyclineExperiment4EffectsofcelluloseOHmicrobialcommunitydiversityinbroilerterminal

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