擬南芥花粉原生質(zhì)體質(zhì)膜陰離子通道的鑒定及其在花粉萌發(fā)和花粉管生長中的作用.pdf

1、中國農(nóng)業(yè)大學碩士學位論文擬南芥花粉原生質(zhì)體質(zhì)膜陰離子通道的鑒定及其在花粉萌發(fā)和花粉管生長中的作用姓名：任永紅申請學位級別：碩士專業(yè)：植物學指導教師：武維華20030601A8S罩RAC善Pollengerminationaswellaspollentubegrowthisanexcellentmodelforthestudyofsignalingmechanismsintipgrowingcells。Inadditiontocalciu

2、mandpotassiumionsthathavebeendemonstratedtoparticipateregulationofpollengerminationandtubegrowth，ithasbeenalsoproposedthatchannelmediatedaniontranspo撞acrosstheplasmamembranemig融beinvolvedintheprocessesThefocusofthisthesi

3、sworkistoidentifyandcharacterizeanionchannelsintheplasmamembraneofArabidopsispollensandtheirsignificanceinregulation0fpoUengerminationandtubegrowthResultsofinvitropollengerminationandtubegrowthshowedthatanionchannelblock

4、ers(DIDS，NFA，NBB羚significantlyinhibitedpollengerminationandtubegrowthTheexternalpHsignificantlyaffectedpollengerminationandtubegrowth，andtheadditionofCI。inthemediumreducedthealkalinepHinducedinhibitionofpollengermination

5、andtubegrowthByapplicationofpatchclamptechniques，thevoltage—dependentinwardcurrentsthatmainlyaccountforanioneffluxwererecorded露’echannelswe∞permeable|。CIandS04。WhenCI’wastheonlychargecarryingioninthepipettesolution，thewh

6、olecellcurrentsshowedrundownrapidlyWhiteCI‘waspartiallysubstitutedwiths04’，therundownofthewholecellcurrentswasnolongerdetectableThewholecellcurrentswereinhibitedbyanionchannelblockerNPPBandalsoinhibitedbyalkalineexternal

7、pH，itisconcludedthatanionlaanspo戢thrc噸】l酶theoutwardanionchannelsidemifiedfromtheplasmamembraneofArabidopsispollenprotoplastsissomehowinvolvedinregulationofpollengerminationandtubegrowthActivityoftherecordedanionchannelsi