24440.蠟狀芽孢桿菌與重金屬鉻相互作用機(jī)制研究

1、福建農(nóng)林大學(xué)碩士學(xué)位論文蠟狀芽孢桿菌與重金屬鉻相互作用機(jī)制研究姓名：陳志申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)藥學(xué)指導(dǎo)教師：黃志鵬林璋20100401福建農(nóng)林：大學(xué)碩士學(xué)位論文AbstractAcomprehensiveunderstandingofthedetailedmechanismisexpectedtohaveasignificantimpactinthedesignoflow—costworkablestrategiesforthetr

2、eatmentandrecyclingofCr(VI)industrialwastesThoughtheinteractionmechanismbetweenCr(VDreducingbacteriaandCr(VI)wasextensivelystudied，howeverforaspecificbacteria，literaturesonlycontainoneortwopointsThecomprehensiveunderstan

3、dingoft11eCr(VI)uptakemechanismofaspecificbacterialstrainisquiteabsentInthisworkwithaCr(VI)tolerantstrainBacillusceYeus∞cereus)asanexample，completeunderstandingofCr(V1)reductionandCr(III)immobilizationmechanismwereolbtai

4、nedForthefirsttime，itWasfoundthattheextracellularsecretionofbacteriacouldplayallimportantroleduringtheCr(VI)reductionandCr(III)immobilizationprocessesProteindenaturantexperimentssuggestednoreducingenzymeparticipatedinthe

5、reductionprocessofCr(VI)bybothextracellularsecretionandcelldebrisofBcereusXrayphotoelectronspectroscopy(xPS)andelectronspinresonancespectroscopy(ESR)analysisrevealedthattheendproductwasCr(IlI)，withCr(V)asintermediateBoth

6、intracellularandextracellularimmobilizationofCr(III)wereobservedbyatomicforcemicroscopy(AFM)，scanningelectronmicroscopy(SEM)andtransmissionelectronmicroscopy(TEM)Inductivelycoupledplasmaopticalemissionspectroscopy(ICP—OE

7、S)analysisrevealedthattheCr(III)dis：tributionintracellularlyandextracellularlyWasabout314％and686％，respectivelyWithFouriertransforminfrared(FTIR)andextendedXrayabsorptionfinestructure(EXaFS)analysis，weconfirmedtheaminoand

8、carboxylgroupsfromthebacterialsurfaceaswellastheextracellularsecretionmightinvolveintheCrimmobilizationprocess，thepossiblecoordinationstructurecouldbesimilartoCr(Glycine)3H20Keywords：Bacilluscereus，chromium，reduction，imm