基于代謝網(wǎng)絡(luò)模型的谷氨酸發(fā)酵在線優(yōu)化與控制的研究

1、分類號◇Ⅺt。罨碩士學(xué)位論文題f=；i1：基于代謝網(wǎng)絡(luò)模型的谷氨酸發(fā)酵在線優(yōu)化與控制的研究英文并列題目：on—lineoptimizationandcontrolofglutamatefermentationusingmetabolicnetworkmodel密級研究生：酆堇專業(yè)：筮醒工程研究方向：筮醛王堂——導(dǎo)師：皇鹽王塾援指導(dǎo)小組成員：段作營副教授學(xué)位授予日期答辯委員會主席：孫志浩教授江南大學(xué)地址：無錫市二oo六年三月江南大學(xué)碩士學(xué)

2、位論文AbstractThispaperstudiedtheoptimizationofglutamatefb珊entationprocessTheexperimentaldatashowedthat，intlleglutarllatefe舯entationbystrainCDr“P6dcfPrf“塒∥甜f口珊fc“肼S9，glut鋤ateproductiona11dlactate(by—product)accurnulationocc

3、urredindiff色rentwayswhencontmllingD0atdifferentleVelsByanalyzingtheexperimentaldatausingthemetabolicne“，orkmodel，wefoundthatthemetabolicnuxshiRedquantitativelySubsequently，enzymaticexperimentswerec枷edouttoVerifythispheno

4、menonTheresultsshowedthat，D0laIgelyef詫ctedglutamatedehydrogenaseactivity(GDH)，ahigherGDHactiVity，aswellastlleoverproductionofbothglutaIIlateandlactatecouldbeachieVedatalowerD0leVelTheoxygenlimitingconditionwasextremelyha

5、礬如1toGDHactiVityandglutamateproductionTherelationshipbetweenDOandRQwasstudied，thedatashowedthatwhenDOwascon訂olledathighleVeltheRQvaluewasllighaIldviceVerSaAcontrolstrategybasedonRQmeasurementwasusedtegulatetheGDHactivity

6、aIldTCAreactionsnuxesaimingforimproVementofthefementationperfoH】1anceAconceptof“BalancedMetabolicControl”wastlluspmposedByanalyziIlgt11etimeresponseofRQtostepchangesinagitationmte，wefouIldthatRQwascontrollableusiIlgtIlea

7、gitationrateastheoperatingV“ableAPIfeedbackcon仃DlIerwasdesignedforregulatingRQandmaintainingmeTCAmetabolicfluxattheappropriatelevel，tooptimizetIleglut鋤atefennentationprocessTheresultsshowed山atbyreducingRQset—pointstep—wi

8、se，themetabolicbalaIlcebe抑eenglycolysisa11dglut踟atesynthesis／TCAcyclecouldbereachedandthelactateaccumulationberepressedWiththiscontr01strategy，thennalglut柚ateconcentrationincreaSed13％andthe1actateaccumulationwasonly64％of