氧化樂(lè)果對(duì)雄性小鼠生殖系統(tǒng)損傷的研究.pdf

1、甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文氧化樂(lè)果對(duì)雄性小鼠生殖系統(tǒng)損傷的研究姓名：徐光翠申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物遺傳育種與繁殖指導(dǎo)教師：鄢珣張波200506012005MasterPaperSUMMARYSUMMARYThispaperstudiedtheeffectoforganiophosphoruspesticide，omethoateonreproductivesysteminmalemicefromthreeaspectswhichare

2、theeffectonthequalityofsperm，ontheactivityofsymbolicenzymesinthetesticleandthedamageonthespermDNAinmice140Kunmingmalemiceweredividedrandomlyinto4groupswhichwererespectivelythecontrolgroup，thelowerdosegroup，themediumdoseg

3、roupandthehigherdosegroupThecontrolgroupwasperfusedstomachlywithO9％NaClsolutionothersweregivenomethoateatvarieddoseof125、25、5mg／kgofbodyweightrespectivelyThenumberactivityandmorphologyoftheirspermsweredeterminedsevenweek

4、safterexposureTheresultsshowedthattheweightofexposuredmiceweresignificantlydecreasedthanofthecontrolgroup，andwiththeincreasingoftheomethoatedose，thetesticleweightofexposuredmiceappeareddecreasingtrendAndtherewaspathology

5、damnificationinthemiceexposuredto5mg／kgofomethoatethatwastesticleshrinking，testicleappearingpurpleandevenafewtesticleshadbeendegeneratedTheratioofthespermsactedwellandthenumberofspermsweresignificantlydecreasedinthemicee

6、xposured25mg／kg、5mg／kgofomethoatethaninthecontrolgroupTheratioofdeadspermsandthespermdeformityweresignigicantlyhigherintheexposuredmicethaninthecontrolgroupAlsothereisallobviousdoseeffectiondependetrelation2Thedifferentg

7、roupsweregivenomethoateatvarieddoseof0、1、2、4mg／kgofbodyweightrespectivelyThesymbolicenzymes(AKPACPLDHandLDH—x)weredeterminedsevenweeksafterexpourResultsshowedthatthebodyweightandthetesticleweightofmicedecreasedobviouslya

8、somethoatedoseincreased，theactivitiesoftheAKPACPLDHinmicetesticlesignificantlyhoisted3Thedifferentguoupsweredonewithomethoateatvarieddoseof05、1、2mg／kgofbodyweightrespectivelyTheDNAstrandbreakageinmicespermweredetermined3

9、2daysafterexposureResultsshowedthatwhenthemicewereexposuredto05、1、2mg／kgofomethoate，therewasnoobviousvarianceinthebodyweight，thetesticleweightandtheratioofthetesticleweighttothebodyweightofmicecomparedwiththecontrolgroup

10、，buttheomethoatehadinjurethespermDNAThereisanobviousvarianceoftheindexofcometcellscomparedwithofthecontrolgroupalsothereisanobviousdose—effectiondependentrelation，F(xiàn)romtheformerstudies，itisconcludedthattherewasanobviousef

11、fectofomethoateonthebodyweightinmiceWhenthedoseis125mg／kgoroverit，theomethoatecanmakethebodyweightofmicelessen；thereis110obviouseffectontheweightoftesticle，theratioofthetesticlewei：ghttothebodyweightforlowerdose(5mg／kg)a