缺血預(yù)處理及后處理對CO-,2-氣腹模型大鼠肝臟缺血再灌注損傷的保護(hù)作用.pdf

1、蘇州大學(xué)碩士學(xué)位論文缺血預(yù)處理及后處理對CO氣腹模型大鼠肝臟缺血再灌注損傷的保護(hù)作用姓名：朱杰申請學(xué)位級別：碩士專業(yè)：兒科學(xué)指導(dǎo)教師：孫慶林20080401缺血預(yù)處理及后處理對C02氣腹模型大鼠肝臟缺血再灌注損傷的保護(hù)作用英文摘要TheProtectiveEffectsofIschemicPreconditioningandPostconditioningonLiverIschemia／ReperfusionInjuryinaRatMo

2、delofC02PneumoperitoneumAbstractObjective：Toinvestigatetheprotectiveeffectsofischemicpreconditioningandpostconditioningonliverischemia／reperfusioninjuryinaratmodelofC02pneurnoperitoneum(P)Method：24maleSDratswererandomlyd

3、ividedinto4groups，pneumoperitoneum口group)，ischemiepreconditioning0Pgroup)，ischemicpostconditioning(IPogroup)andcontrol(Cgroup)Cgroupwassubjectedtoshamoperation。OthergroupsweresubjectedtotheC02pneumoperitoneum，15mmHgintra

4、—abdominalpressure(IAP)PgroupWassubjectedto60minofP，followedby30minofdeflation(D)IPgroupWassubjectedtopreconditioningpriortoP／D，whichconsistedof10minofP，followedby10minofDIPogroupwassubjectedto60minofP，followedbythreecyc

5、lesof1minofDand1minofPand30minofDPlasmaalanineaminotransferase(ALT)andaspartateaminotransferase(AST)，aswellashomogenizedtissuemalondialdehyde(MDA)andnitricoxide(NO)levels，glutathione(GSH)andsuperoxidedismutase(SOD)activi

6、tiesweremeasuredrespectivelyThehepaticpathologicalchangeswerealsoobservedbylightmicroscopyTheexpressionofinduciblenitricoxidesynthetase(iNOS)inlivertissuewereexaminedbyimmunohistochemicaltechniqueineachgroupResults：Plasm

7、aALTandASTaswellasliverMDAlevelsweresignificantlyincreased，whereasliverSODvaluesweredecreasedingroupsP，IPandIPo，ascomparedtogroupC儼005)PlasmaALT，ASTaswellasliverMDAandNOlevelsweresignificantlydecreased，whereasliverSODand