內(nèi)蒙古絨山羊與蒙古綿羊BMP-2基因片段的克隆及表達(dá).pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文內(nèi)蒙古絨山羊與蒙古綿羊BMP2基因片段的克隆及表達(dá)姓名：趙珺申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物遺傳育種與繁殖指導(dǎo)教師：李金泉尹俊20060501CloningandExpressionofBMP一2GeneFragmentsfromArbasCashmeregoatandMongoliasheepAbstractThecashmerecomesfromthesecondhairfollicleofCashmerego

2、atThequantityandqualityofcashmeredependonthestructuresandcharacteristicsofthehairfollicledirectlySomesignalmoleculehaverelationstothedevelopmentandcirclingthegrowthofhairfollicle，It’SreportedthatBMP一2／4controledbyNogginw

3、hichisoneofthesignalmoleculehasrelationtothesecondhairfollicleofrat，butnoneofthereportsabouttherelationshipofBMP一2withCashmeregoatandMongoliasheepThegenomeDNAwereextractedfrombloodinArbascashmeregoatandMongoliasheepThese

4、quencewereobtainedbyPCRandclonedintopMDl8TvectorThepositiveclonesweresequencedFinallywecomparedthesequencesofBMP一2genefragmentsamonggoats，sheepandotheranimalsthathavebeensubmittedtoGeneBank觸igmentofthesequenceofArbascash

5、meregoat，Mongoliasheepwithotheranimalsshowedthattherewas99％nucleotideidentitybetweenArbascashmereandMongoliasheep，87％identitybetweenCashmereandHuman，85％identitybetweenCashmereandMouse99％identitybetweenCashmereandCattle，8

6、6％identitybetweenCashmereandDog；onthebasisofcomparingthesheepsequencetoHuman，Mouse，CattleandDog，thefindinghomologywere87％，85％，99％85％ThenwemadetheprobewithDIGusingthecloningproductofcashmeregoatandMongoliasheep，forstudyin

7、gtheexpressionofBMP一2inhairfollicleofArbascashmeregoatandMongoliasheepby／nsituhybritionIncashmeregoat，wefoundthegreatexpressioninkeratinizationcelloftheprimaryhairfolliclein75days，thegreatexpressionintheprimaryandtheseco

8、ndhairfollicleof115days；TheexpressioninIRSattheprimaryhairfollicleinMarch，theexpressioninthesecondhairfollicleinMaytheslightexpressionintheprimaryhairfollicleinOct；TheexpressioninfirsthairfollicleinMongoliasheepinNovKeyw

9、ords：Cashmeregoats；Mongoliasheep；BMP2；colone；InsituhybridizationDirettedby：ProfDrLIdinquan(PhD)AssocaitedProfDrYlNdunAppIicantforMasterdegree：Zhaodun(Ani=alGeneticsandBreeding)(AnimalScienceaⅡdAmmalMedicine，InnerMongolia