微流控單分子檢測免疫分析技術(shù)的初步研究.pdf

1、山東大學(xué)碩士學(xué)位論文微流控單分子檢測免疫分析技術(shù)的初步研究姓名：段煜洲申請學(xué)位級(jí)別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：張曉麗20080527山東大學(xué)碩士學(xué)位論文ABSTRACTThethesisincludesthreeparts：IThepreliminarystudyoftheconditionsofmicrofluidicimmunoassaybysinglemoleculesdetectionIITheoptimizationandc

2、hoiceoftheconditionsofmicrofluidicimmunoassaybysinglemoleculesdetection1IIDetectingconditionsofCancerantigen125singlemoleculesInchapteroneofthisdissertation，abriefreviewofmicrofluidic，immunoassayanalysisandsinglemolecule

3、sdetectionwasmadeInthispart60referenceshavebeenreferredThenthepreliminarystudyoftheconditionsofmicrofluidicimmunoassaybysinglemoleculesdetectionWaSmadeSeveralparametersincludingmodificationofPDMS，silanizationtime，driving

4、voltage，buffersystemwereoptimizedThefollowingconditionsweresuitableformicrofluidicimmunoassaymodifiedPDMSslabwithnaScentoxygenfor2days；theglasscoverslipwassilanizedwith3％GOPSfor24h；bufferwashingconditions：injectionvoltag

5、e，1000Vinjectiontime，20min；blockingconditions：injectionvoltage，1000Vinjectiontime10S，quiettime50S，repeatthisprecess：80times；incubationconditionsinjectionvoltage，1000Vinjectiontime10S，quiettime50S，repeatthisprecess：80time

6、sInchaptertwoofthisdissertationwemadeafurtherinvestigationaboutthemicrofluidicimmnunoassayconditionsonthebaseofchapteroneThediameterofthereservoirs，thefabricationanddimensionofthemicrochannelswerechangedAllchannelsonthe～

7、5mmthicknessPDMSslabwithalengthof4cmandawidthof2cmwere150pmwideand～20pmdeepOnthischipweinvestigatedthewashingconditions，blockingtimeandincubationtimeetcInchapterthreeofthisdissertation，weinvestigatetheconditionsofsandwic