L-鳥氨酸的分離純化工藝研究.pdf

1、南京工業(yè)大學碩士學位論文L鳥氨酸的分離純化工藝研究姓名：朱慶平申請學位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：萬紅貴200706碩士學位論文ABSTRACT1漲嬲Y2084086L一0mimiIleis吼intennediatemetaboliteiIla瑪illillealldci饑lllillebiosyllthesisItise舵ctiveilltlle仃eatmentofliverdisorderSSoithasasizablewor

2、ldmarkethl也ispaper，thefe吼entationbromofLomithinewaSpre廿eatedbymembraneseparationtecllIlolo盯TheseparationconditionofLomimillebymeallsofSilIlulatedMoVillgBedtecllllologywaSstIldied1hedecolorationa11dc巧stallizationcondition

3、sofL—omiminewerealsostudiedItis吐1ebaSedresearchforproduceL咖imiIleinindus仃yTheceramicmembr鋤ewitllmolecularweightcut—off500kDwaSselectedforitsfluxand也ereSultofremovalbiomassTheefrectofdi仃erentconditions0nmembranenuxwasi11V

4、estigatedThe0ptimaloperationconditionsofmembraneseparationoft11efementationbrothofLomimineareasfollow誼g：pHis25，presSureisO22MPa，temperatureis60℃，alldconcen仃ationratiois14。Thea皿moniatyperesiIlLH6waSselectedbythestaticadSo

5、rptiontest11uough也ed)，11鋤icteSt，the叩timal0perationconditionsareaSfollowiIlg：theconcen廿ationofL一0mithineis309／L，hei曲t／di鋤eterratioofbedis59，pHis20，feedrateis1OmL／min，theeluentisO5mo兒鋤monia，eluentrateis1OmL／mill。Theconcent

6、rationofLomitllineinex打actportandramnateportofSMBsystemwasinVestigatedThe叩tiIIlaloperationconditionsare髂followiIlg：feedrateis258mL／min，washingrateisl73mUmin，eluentrateis260mL／min，cleanrateis345mUmin，switchtimeis290hTheyi

7、eldofLomithilleis乎eater也aIl97％in也eoptiIllaloperationconditionsSMBsyStemisabletostablenm，ttleyieldofLomi血inebySMBsyst鋤is黟eaterth鋤siIlglecoluI】[111s印amtion，aIldaIIⅡnoniacons咖1ptionbySMBsystemis10wermansiIlglecolu玎ms印刪ionhl

8、meopt蛔alconditions，theyieldofcmdeL—omithinec穢StalisgreaternlaIl80％，medecolorratioofLomi也iIleis盟eatert11aIl95％Thec巧stalIizationconditions0fLomi吐liIlewereillVestigatedTheoptinlalcryStallizationconditionsareaSfollowing：conc