豬應(yīng)對運(yùn)輸應(yīng)激的品種特征及腎上腺皮質(zhì)功能差異的分子機(jī)制.pdf

1、中文摘要豬應(yīng)對運(yùn)輸應(yīng)激的品種特征及腎上腺皮質(zhì)功能差異的分子機(jī)制摘要本文通過比較運(yùn)輸過程中二花臉豬和皮特蘭豬的行為和血液生理生化指標(biāo)的動(dòng)態(tài)變化，以客觀評價(jià)兩品種豬的應(yīng)激敏感性，并在此基礎(chǔ)上，提出血漿皮質(zhì)醇水平的品種差異與腎上腺皮質(zhì)功能差異有關(guān)的假說為驗(yàn)證這—假說，本研究從體內(nèi)和體外兩方面，對豬腎上腺皮質(zhì)細(xì)胞皮質(zhì)醇合成相關(guān)信號通路進(jìn)行比較研究，在腎上腺皮質(zhì)水平揭示了不同品種／基因型豬血漿皮質(zhì)醇水平差異的分子機(jī)制1運(yùn)輸過程中二花臉豬和皮特蘭豬

2、行為、血漿酶活和激素水平的動(dòng)態(tài)變化本實(shí)驗(yàn)采用實(shí)時(shí)跟蹤攝像和頸靜脈血管瘺連續(xù)采樣，觀察二花臉豬(Har啉)和皮特蘭豬(ml肌)在2h運(yùn)輸過程中的行為表現(xiàn)，并監(jiān)測血漿肌酸激酶(CK)’乳酸脫氫酶(LDH)活性以及應(yīng)激和代謝相關(guān)激素水平的動(dòng)態(tài)變化結(jié)果表明運(yùn)輸前期(出發(fā)后15min)二花臉豬主要表現(xiàn)探究行為和異常行為，中后期(出發(fā)后60—75rain和最后15min)其蹲坐和躺臥行為逐漸增加；相反，皮特蘭豬在運(yùn)輸前期探究行為較多，中后期主要表現(xiàn)

3、為站立行為運(yùn)輸過程中皮特蘭豬血漿CK(P001)和LDH活性(戶c005)均顯著高于二花臉豬。血漿CK活性呈現(xiàn)顯著的時(shí)間效應(yīng)和時(shí)間與品種的交互作用(P(001)血漿ACTH水平在運(yùn)輸過程中顯著升高(P005)，但品種間差異不顯著血漿皮質(zhì)醇水平在運(yùn)輸開始后15min即顯著升高(P‘005)，上車前及整個(gè)運(yùn)輸過程中二花臉豬皮質(zhì)醇水平均顯著高于皮特蘭豬(Pc005)代謝相關(guān)激素(胰島素、13和T4)在運(yùn)輸過程中總體表現(xiàn)下降，呈顯著的時(shí)間效應(yīng)(

4、P001)，但均未表現(xiàn)顯著的品種差異以上結(jié)果提示：不同品種豬在運(yùn)輸過程中表現(xiàn)不同的行為模式和血液生化指標(biāo)的變化；二花臉豬和皮特蘭豬血漿ACTH水平無顯著差異，而皮質(zhì)醇水平品種差異顯著，提示兩品種豬腎上腺皮質(zhì)功能存在差異2豬腎上腺皮質(zhì)醇合成途徑的品種差異及其分子機(jī)制為揭示二花臉豬和皮特蘭豬腎上腺皮質(zhì)功能的差異，本研究通過在mRNA和蛋白質(zhì)水平檢測兩品種豬腎上腺黑皮素受體2(MC2R)、cAMP反應(yīng)元件結(jié)合蛋白IIIBREEDSPECInC

5、STRESSCOPINGCHARACTElUSTICSINPIGSDU砒NGTRANSPORIANDMOLECULARMECHANISMSUNDERI肌NGTHEBREEDDIFFERENCESINADRENOCOI婦[1CALFUNCTIONABSTRACTThisstudywasaimedtoanalyzethebreed—specif／ccharacteristicsofpigsinresponsetotransportstres

6、s，throughcomparisonofthedynamicchangesofbehaviorplasmaphysiologicalandbiochemicalmeasuresbetweenErhualian(Elm)andPietraln(PIE)pigs，during2hoftransportationInordertotestifythehypothesisthatthebreeddifferenceinplasmacortis

7、ollevelsisofadrenocorticalorigtheexpressionpatternofproteinsinvolvedinadrenalACTHsignalingpathwayincludingMC2RCREBandpCREB，STARaswellasthatofthekeyenzymesinvolvedinsteroidogenesisinEHLandPIEpigsWascharacterizedbom／nvivoa

8、nd／nv／tro1DynamicchangesofbehaviorplasmaenzymesactivitiesandhormonelevelsinErhualianandPietrainpigsduringtransportEHLandPIEpigsequippedwithchroniccathetersinjugnlarveinwercsubjectedto2htransportBehavioralexhibitionofthep

9、igsWasrecordedwithavideocameraanddynamicchangesoftheactivitiesofcreatinekinase(c趵andlactatedehydrogenase(tam)，aswellastheplasmaconcentrationsofstressandmetabolichormoneswereinvestigatedinEHLandPIEpigsduring2htransportThe

10、majorityofthebehaviorofEHLpigsconsistedoforal／nasal／facial(ONE)andfitbehaviorsduringtheinitialobservations(first15minafterstart)，andthesebehaviorswerereplacedbyincreasedtimesittingandlyinginlaterobservations(middleandlas

11、t15milloftransport)IncontrastPIEpigsshowedhighlevelsofONFbehaviorsininitialobservationfollowedbyhigh矗equencyanddurationofstandingduringthemiddleandthelastobservationpedodPIEpigsdemonstratedsignificantlyhigherplasmaCK伊001

12、)andLDHactivities耿005)ThereweresignificanteffectsoftimeandtimeXbreedinteraction011CKactivities(Po01)inbothbreedsPlasmaACTHlevelsdidnotdifferbetweenbreeds，yetasignificanteffectoftime(P005)WasshownduringtransportPlasmacort