北京地區(qū)茄子枯萎病原菌鑒定及抗病種質(zhì)資源篩選.pdf

1、中國(guó)農(nóng)業(yè)科學(xué)院碩士學(xué)位論文北京地區(qū)茄子枯萎病原菌鑒定及抗病種質(zhì)資源篩選姓名：陳鈺輝申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：連勇20071201AbstractAccordingtotheMOAstatisticdatain2005，thevegetableareasofchinaare2658105miIlionRlUWiththeincreaseofeggplantplantingareasyearbyyearthekindsofeg

2、gplantdiseasesandpestsalemoreandmoresevereBesideseggplantverticilliumwilt，bacterialwiltandphomopsisblightetc，whichbringtheseriousdamageofeggplantproduction，inrecentyeartheeggplantfusariumwiltappearandaresevereyearbyyearI

3、nsomeofplace，theincidenceofadiseasenormallyare1040％，thereducationofoutputisabout10％andanyspecialyeararrived20％or100％inseriousdiseaseareasBecausethesymptomsofeggplantfusariumwiltaresimilarwitheggplantverticilliumwiltwhich

4、isearlybeenreportedandresearched，andsometimestwodiseaseshappenedtogetherthepersonrefertOpayattentiontoverticilliumwiltandneglectthefusariumwiltUntilnowinourcountlnjtheredidnothavethereportandresearchoneggplantverticilliu

5、mwiltpathogen，methodofartificalresistanceidentificationonseedlingstage，andresistantidentificationofeggplantgermplasmTheteststartswithseparationandidentificationofpathogen，andthensystemresearchonbiologicalcharacteristics，

6、methodofartificalresistanceidentificationonseedlingstage，andresistantidentificationofeggplantgermplasmTheresultsshow：1theisolatedpathogenfromwilteggplantingreenhouseofBeijingareasisFusariumoxysporumSchlthetestprovedthatt

7、hewiltandyellowleaveseggplantinBeijingareasaredamagednotonlybyVerticiUiumdahlia，butalsobyFusariumoxysporum2thestudyonbiologicalcharacteristicsshows：forFusariumoxysporumSchl，theoptimumculturemediumisPDA；theoptimumcarbonan

8、dnitrogensourceweresugarandpeptone；theoptimumgrowthtemperatureisabout2530(2；theoptimumgrowthPHis7theoptimumsporegerminationtemperatureisabout2030℃；thegerminationratearrives80％after12h；theconidiosporelethaltemperatureis50

9、“(2with15min3ToanalysethedatabyusingSASsoftwareandscreeningofmethodofartificalresistanceidentificationonseedlingstage，theoptimumcombinationis：dippingrootinoculation，inoculationconcentrationlxl07spore／ml，3trueleavesseedli

10、ngstage4Screening23eggplantresistancegermplasm14eggplanttolerancegermplasm，3eggplantsensiblegermplasmTheresultshowstherearcdonothavehighresistancematerialswithlowdiseaseindexesThetestsystematicallystudyedonpathogenandits

11、biologicalcharacteristics，themethodofresistantidentification，atsametime，screeningtheeggplantgermplasmwhichholdinourresearchgroupTheresultlayafoundationforeggplantresistantFusariumwiltbreedingKeyword：eggplant，F(xiàn)usariumoxys