甘藍型油菜隱性核不育系20118A的遺傳解析、定位與小孢子選育.pdf

1、上海交通大學博士后學位論文甘藍型油菜隱性核不育系20118A的遺傳解析、定位與小孢子選育姓名：楊立勇申請學位級別：博士后專業(yè)：油菜分子生物學指導教師：羅利軍孫超才20080401AbStractmthepresentpaper20118AB，ahomozygousGMStwo—typelineinBrassicanapus，iSusedasoneoftheparenttocrosswith16Brassicanapusaccession

2、stostudy：geneticanalysisoftherecessiveGMS；fertilityperformanceoftherecessiveGMSundervariousgeneticbackground；geneticmappingoftheinhibitorgene(忉Mainresultsofthepresentstudiesareasfollow：1Theresultsoftest—crossingwith16Bra

3、ssicanapusaccessionsindicatedthattherestorerlinesofrecessiveGMS20118Awerecomprehensive；itssterilitywasstableandcompleteunderdifferentgeneticbackground，butdeadbudsofthesterilelineshadasignificantdifferenceundervariousgene

4、ticbackground2Geneticanalysisof20118ABshowsthatthegenotypeofthesterileplantsf20118A)isaabbRtRfandthatofthefertileplants(20118B)isAabbRfRforaaBbRfRf；temporarymaintainerlinecouldmaintainthesterilityofvarioussterilelineswit

5、hthegenotypeofaabbrfif3IntheBClpopulationof[(20118Ax“M一6029”)x“20118B”】，AFLPtechnologyinconjunctionwithbulkedsegregantanalysiswereperformedtoidentifygeneticmarkersfortherecessiveepistaticinhibitorgene(r0Fromthesurveyof51

6、2pairofAFLPprimers，7markerscloselylinkedtotherfgenewereobtainedFiveofthemwereflankingtherfgeneononeside，theothersresidingattheoppositeside，andthefragments(EAl0MC07、EAl0MC03)weretwomostvaluablemarkerswiththeclosestgenetic

7、distancesof4cM、3cM，respectivelyEA10MC03markerwasconvertedintoSCARmarkerwhichiseasytobeusedinbreeding4TheembryoswereobtainedfromtweciprocalcrossesF1bytheisolatedmicrosporeculture，itwouldprovidanewwaytodevelopandselecttemp

8、orarymaintainerlinewiththreepairofrecessivegenes5Anewprotocolwasestablishedtoidentifyunknowntargetsequencesadjacenttoknownsequences，anditwasusefultoPCRwalkingofsmallknownsequenceKeywords：BrassicanapusL；recessivegeniemale