基于超氧化物歧化酶的生物傳感器的研究.pdf

1、嗵781830碩士學位論文(2005屆)基于超氧化物歧化酶的生物傳感器的研究StudiesofbiosensorsbasedOnsuperoxidedismutase研究生姓名——塹坐坐——指導教師姓名狄俊偉教援專業(yè)名稱廛旦垡堂研究方向——至些金塹論文提交日裁一——2QQ三主壘魍———AbstractThreekindsofnovelsuperoxidedismutase(SOD)electrodes(SOD／Cys／solgel，SO

2、D／Cys／AuNPandSOD／Cys]AuNP／sol—gel)weredevelopedbycombiningselfassembledmonolayers(SAMs)，nanotechnologyandsol—geltechnologyTheeffectofpHandscanrateonthepeakcurrentandpeakpotentialofSODelectrodesandtheirstabilitywerestudie

3、dsystematicallyThepreparationconditionswereoptimizedTheSODelectrodeswereusedtorespondtosnperoxideanion，whichwaspreparedbyDMSO／NaOHandxanthine／xanthineoxidnsesvstemsThefasterelectrontransferrateofSODelectrodeswitIlhighers

4、ensibilityandexcellentstabilityinthepresenceofLcysteineandcatalysisofAunanoparticleswastestifiedThesol—gelfilmcallnotonlyimmobilizeSODefficientlybutalsomaintainitsreactivitywellThecurrentratioofthecathodicpeakcurrenttoth

5、eanodiconeoftheSOD／Cys／AuNP／sol—gelelectrode(ipc／ipa=11at100mVsq)andtheseparationbetweentheanodicandcathodicpeakpotentials(△Ep_Epa—EPcO24Vat100mVs“)indicatethattheelectrodeprocessofSODisquasi—reversibleTheformalpotential

6、，estimatedas(E礦Epc)／2，was1IOmV(versusSeE)Theheterogeneouselectrontransferrateconstantwasevahmtedtobeabout103s一Thereactivityofelectrode，whichwasstoredaftersixtydays，maintained97％MakinguseofgoodqualitiesoftheSAMs，nanotechn

7、ologyandsol—geltechnologythesuperoxidedismutaseimmobilizedbythinsilicasolgelfilminthepresenceofL—cysteineandAunanoparticlesongoldelectrodesurfacecanbedevelopedwithhighersensibilityandexcellentstabilityKeywords：Superoxide