克雷伯氏菌發(fā)酵生產(chǎn)1,3-丙二醇的研究.pdf

1、大連理工大學(xué)碩士學(xué)位論文克雷伯氏菌發(fā)酵生產(chǎn)13丙二醇的研究姓名：王劍峰申請學(xué)位級別：碩士專業(yè)：生物工程指導(dǎo)教師：修志龍范圣第20000601Abstract大連理工大學(xué)碩士論文ABSTRACTInthispaper，atitrationmethodwasestablishedbywhichglycerincontentcanbemeasured，comparedwithenzymemethodandspectrophotometricm

2、ethod，Itshowedthatthemethodwasexactenoughforglycerinanalysis，andwaseasytooperateInthispaper，theculturemediabyKlebsiellapneumonoewasoptimizedat2％4％and6％ofinitialglycerinconcentrationItshowedthattheculturemediaoptimizedwas

3、morefittedtoindustrialapplicationduetoitslesscomponencesthanreportedinliteratures，andahighyieldwasobtainedDuringtheoptimization，theinfluencesofthecationsNa，K，NH4ontheyieldofglycerinto1，3propanediolwereinvestigatedItappea

4、redthattheyieldof13一propanediolwaslowiftheconcentrationofthecationNa‘intheculturemediawashigh，andwhereshighiftheconcentrationofthecationNH^washighinthepresenceoflowconcentrationofKInthispaper，bioconversionofglycerinto1，3

5、propanediolbyKlebsiellapneumonaewasstudiedunderaerobicandmicro—aerobicconditioninbatchfermentationat2％，4％and6％ofinitialglycerinconcentrationAsaresult，thecellscangrowandyieldthepuductssuchas1，3propanediol，aceticacid，ethan

6、olunderaerobicandmicroaerobicconditionAndaceticacidwasmuchthanunderanaerobicconditionbutethanolless，andtheyieldof1，3一propanedi01betterat2％，4％ofinitialglycerinconcentrationundermicroaerobicconditionItshowedtheenzymesrespo

7、nsibleforthesynthesisof13propanediolhadn’tbeeninactivedbyoxygeninbatchfermentationandmicro—amountofoxygencallcontributetoconversionofglycerint01，3propandi01Inaddition，aftercontinuedfermentationhadbeencarriedoutfor96hrund