里氏木霉與黑曲霉混合發(fā)酵產(chǎn)纖維素酶的研究.pdf

1、合肥工業(yè)大學(xué)碩士學(xué)位論文里氏木霉與黑曲霉混合發(fā)酵產(chǎn)纖維素酶的研究姓名：高星星申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：食品科學(xué)指導(dǎo)教師：潘麗軍201204StudyoncellulaseproductionbymixedfermentationofTrichodermaresseiandAspeABSTRACTLignocelluloserawmaterialsderivedfromcropstrawiSrichandcheaprenewablereso

2、urcesonearth，WhichishydrolyzedtoconvettomixedsugarcontainingglucoseandxyloseThemixedsugarisoneofthepotentialandimportantbiologicaltransformationmaterialsTheeffectivedevelopmentanduseofthesecellulosicfeedstocksiStheeffici

3、entpathtorelieveenergycrisisanddecreasethegrainforindustryInthisstudy，astheoriginalstrain，TrichodermareeseiismutatedbyCooo丫一rayHighyieldcellulasestainwasbreededandscreened；Thenresponsesurfacemethodwasusedtooptimizethecon

4、ditionsofmixedfermentationofTrichodermareeseimutantsandAspergillusniger；Finally，theeffectofcelluloseenzymatichydrolysisoflignocellulosicrawmaterialswasstudiedThemainconclusionsareasfollows：1)ThemutanteffectofCo叫丫一rayonTr

5、ichodermaresseiwasverywellAfterrepeatedmutagenesisunderoptimalmutagenicdose400Gy，34strainswereobtainedThroughcellulosecongoredmediumscreeningandshakeflaskre。screening，ahigh‘yieldcellulasestrainwasobtainedThefilterpaperac

6、tivityofthemutantwas1170IU／mL，increasingby1058％thantheoriginalstainHereditarystabilitytestdemonstratedthattheproductivitiesofmutantfrom1stto5thserialpassagewerestable2)Thefermentationconditionsforenzymeproductionbymixedf

7、ermentationwereoptimizedbyBox—BehnkenresponsesurfacemethodologyTheregressionmodelwasestablishedusingDesignExpertTheoptimalenzymeproductionconditionwere：Anigerdelayvaccinationtime36h，TrichodermareeseiandAspergillusnigerin

8、oculationratioof5：l，thefermentationtime6d，thesolid1iquidratio2：50(g／mL)，theamountofTween804mL／L，pH50，liquidvolumeof250mLshakeflask50mLInthisconditionthefilterpaperactivitywas1224IU／mLandthepglucosidaseactivitywasO315IU／m

9、LTheproofexperimentprovedthatthefilterpaperactivityfailedwithinthe95％responsepredictionintervalrange，whichshowedthattheregressionmodelcoulddescribetherealenzemeproductionsituationwell3)Thefactorswhichinfluencedtheeffecto

10、fcrudeenzymeenzymatichydrolysiswereexploredThebestenzymatichydrolysisreactionconditionswas：cellulasehydrolysistemperature50“C，theoptimumpH5，theoptimumhydrolysistime5hInthiscondition，crudeenzymewasusedtoenzymatichydrolysi