表達(dá)兔出血癥病毒VP60蛋白重組干酪乳桿菌的構(gòu)建及其免疫學(xué)初步分析.pdf

1、ClassifiedIndex:U.D.C:DissertationfortheMasterDegreeinAgricultureCONSTRUCTIONOFRECOMBINANT五注C廠OB且C幾五以夕C月漢盯EXPRESSIONVP6OOFRABBITHEMORRHAGICDISEASEVIRUSANDIMMUNOGENICITYANALYSISCandidate:ZhangyiSupervis:prof.Li腸一ingAeadem

2、icDegreeAPPliedfor:MasterofAgricultureSPeeiality:PreVentiveVeterinaryMedicineDateoforalExamination:May，2010University:NtheastAgriculturalUniversity東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文2.2.4重組干酪乳.桿菌的構(gòu)建...........................................

3、.............................................……242.2.5重組干酪乳.桿菌的誘導(dǎo)及表達(dá)蛋白的鑒定...............……，..................................……262.2.6口服重組干酪乳桿菌免疫學(xué)指標(biāo)的測定……，.....................................................……282.2.7免疫兔攻

4、RHDV保護(hù)性試驗(yàn)...............................................................................……293實(shí)驗(yàn)結(jié)果..........................................................................................................................…

5、…，.…303.1RHDV的鑒定................................................................................................................……303.1.1敏感兔接種試驗(yàn)............................................................................

6、........................……303.1.2HA和Hl試驗(yàn)..................................................................……，.............……，..........……303.2病毒VP6O基因的RYPCR................................................................

7、...........................……303.3VP60基因的序列測定及生物信息學(xué)分析...................................................................……303.4重組質(zhì)粒pMD18一TVP60的鑒定結(jié)果........................................................................…

8、…313.5重組乳酸菌表達(dá)載體的鑒定.................................................................................……，.……引3，5.1表面表達(dá)型重組載體PPG一1一VP60的鑒定結(jié)果.................................................……313.5.2分泌表達(dá)型重組載體PPG一2一VP60的鑒定結(jié)果...

9、..............................................……353.6重組干酪乳桿菌的誘導(dǎo)及表達(dá)蛋白的鑒定.................................................................……353.6.1細(xì)胞表面表達(dá)型重組干酪乳桿菌的誘導(dǎo)表達(dá)鑒定結(jié)果二_...............……、..........……353.6.2分泌表達(dá)型重組干酪乳桿菌的

10、誘導(dǎo)表達(dá)鑒定結(jié)果............................................……373.7重組乳酸菌口服免疫兔免疫學(xué)指標(biāo)的測定.................................................................……393.7.1EuSA方法檢測免疫兔血清中抗RHDVVP60特異性抗體IgG測定............……393.7.2應(yīng)用血凝抑制試驗(yàn)檢測免疫兔的血

11、清效價(jià)........................……，.........................……403.7.3家兔糞便中抗明DVVP60特異性slgA檢測結(jié)果.....................................……，..…403.7.4家兔鼻洗液中抗朋DVVP60特異性slgA檢測結(jié)果.......................................……403.7.5免疫兔攻RHDV保

12、護(hù)性試驗(yàn)...............................................................................……404討論......................................……，........................................................................................…

13、…444.1兔病毒性出血癥病毒09一SD株衣殼蛋白的遺傳變異分析.........................................……444.2口服重組干酪乳桿菌誘導(dǎo)的體液免疫應(yīng)答................................................……，...........……454.3口服重組干酪乳桿菌誘導(dǎo)的免疫保護(hù)作用...................................

14、..............................……464.4表面表達(dá)和分泌表達(dá)誘導(dǎo)免疫應(yīng)答的差異分析.........................................................……475結(jié)論......................……，..........................................................................

15、...............................……49致謝......................................................................................................................................……50參考文獻(xiàn)..........……，............................

16、......................................................................……，........……51附錄...................................................................................……，...............................................