雛雞感染毒害艾美耳球蟲后血液和免疫器官免疫變化及機(jī)理.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文雛雞感染毒害艾美耳球蟲后血液和免疫器官免疫變化及機(jī)理姓名：劉晶申請學(xué)位級(jí)別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：鄭世民20040601ImmunologicChangesandM幽羽li鋤ofPeripheralBloodandImmuneOrgansinChicksInfectedwithEimerianecatrixABSTRACTInthisstudystaphytococcalproteinA(SPA)rose

2、鍵etestindirectenzymelinketimmunosorbentassay(豫LISA)andimmunohistochemistryABCwereusedtesearchthedynamicchangesofthenumberofTorBlymphocytesandthecontentofimmunoglobulin(Ig)G，IgM，leainpcripheralbloodthenumberofCD4’，CDslymp

3、hocytesubpopulation，TANAEcellsandIgG，IgMIgAantibodyproducingcells，theratiosofCD‘’andCDsTcallsinimmuneorgans(thymus，bursaofFabrieiusandspleen)ofchicksprimaryandsecondaryinfectedwithEimerianecatrixfEnecalrix)respectivelyin

4、ordertorevealmechanismofprotectiveimmunityofchicksinfectedwithEnecatrixandofferimportanttheoryfordevelopmentcoccidiosisvaccineTheresultswergasfollows：④髓enumbersof鼉Blymphocytesandthecontentofl謇G。lgM，leainperipheralblood，t

5、henumberofCD4，CDslymphocytesubpopulation，1knEcellsandIgG，IgM，leaantibodyproducingcells，theratiosofCD4andCDsTcellsininlmurleorgansofchicksprimaryinfectedwith置necatrixwereincreasedcomparedwithcontrols7~2ldayspostinfection(

6、班)indifferentdegree。Thenumberincreasedsignificantlyinapeakaround14～8daysPITheobservationsshowedtllatcellularandhumeralimmunefunctioninperipheralbloodandimmuneorangesofchicksprimaryinfectedwithEnecatrixwereenhanced②Thenum

7、bersofLBlymphocytesandthecontentof越G，gM，leain辨療曲eralblood，thenumberofCD：，CDslymphocytesubpopulation，k戚’callsandlgG’IgM|醛antibodyproducingcellsinimmuneorgansofchickssecondaryinfectedwithEnecatrixwe懈decreasedcomparedwithpr

8、imaryinfectedchicks27daysPIindifferentdegreeThereafterth舛begintoincrease。Thenumberincreasedsignificantlycomparedwithcontrolsandprimaryinfectionchicks10～14daysPI。熟eresultsindicatedthatimmunefunctionofchickschallengedwithh

9、ighdosessporulatedoocystswasdecreasedtransitorybutthechickswerewellprotectedbecauseofcellularandhumoralimmunelevelsexcitedbyprimaryinfectedOntheotherhandcellularandhumeralimmunelevelsofchickssecondaryinfectedwithEnecatri

10、xwereincreasedsignificantlyinlatertimewhichindicatedthatimmunerecallresponsewascameintobeing③Thecellularimmunelevelwasearlierenhancedthanthehumeralimmunelevelinchicksinfectedwith點(diǎn)1necatrixTheresultsindieatedthatcellulari