單個量子點的光學性質研究及其在超高分辨率定位上的應用.pdf

1、湖南大學博士學位論文單個量子點的光學性質研究及其在超高分辨率定位上的應用姓名：石星波申請學位級別：博士專業(yè)：分析化學指導教師：楊士成；蓋宏偉201206單個量子點的光學性質研究及其在超高分辨率定位上的應用AbstractAsnovelfluorescentnanomaterials，quantumdotshaveplayedimportantrolesinmanyfileds，suchaschemicalanalysis，biology

2、sensor，molecularimaging，owingtotheirexcellentopticalpropertiesStudiesonthepropertiesofsinglequantumdotcanhelptofindnewexperimentalphenomenology，whichcan’tbefoundintheensembleapproach；provideamentalitytoimprovetheproperti

3、esofquantumdots；contributetOabetterapplicationofQDsinvariousfieldsUtilizingthewidefieldfluorescencemicroscopyasthemainstudyapproachandtakingsingleQDasresearchobject，thisdissertationshowedphotobleachingofQDsbynonexcitatio

4、nlight，presentedtheinfluencefactorofoxidationprocess，andappliedtheQDstosinglemoleCulecountingandsuperresolutionlocalizationThemainworkwereasfollows：1PhotobleachingofQuantumDotsbyNonexcitationLightCore／shellquantumdotsare

5、foundtobephotobleachedbynon‘excitationwavelengthlightatthesinglemoleculelevelandensemblelevelThatmeanseventheprovidedphotonshavenotenoughenergytopumpelectronsintoconductiveband，thephotoreactionofQDforphotobleachingstillo

6、ccursbutwithslowerratecomparmgwithphotobleachingbyexcitationlightThephotodynamicofphotobleachingunderthenonexcitationilluminationalsofollowstheconsecutiveelementaryreactionsasthebehaviorofexcitationwavelengthirradiation2

7、OxidationDynamicofSingleCdSe／ZnSQuantumDotsQDsphotospectalblueshiftarecausedbyanoverallsizereductionoftheQDsduetophotooxidationInthischapter，weutilizedthespectralimagingtomonitortheprocessofbuleshift，thatequalstomonitort

8、heQDsoxidationprocessAfterinvestigatedtheinfluenceofexcitationwavelenghandintensityonCore／shellCdSe／ZnSqauntumdotsphotospectralblueshift，wefoundthattheextentoftheblueshiltstronglydependsontheexcitationwavelengthandQDsini

9、tialsize，butnotdependsontheexcitaionintensityThecoreoxidationrateisalmostproportionaltotheexcitationintensity，shorterexcitationwavelengthinducesfasteroxidationrate3AmeathodformeasuringextentofQDsaggregationRealtimemeasur