杏鮑菇液體發(fā)酵及多糖對小鼠脾淋巴細胞增殖作用的研究.pdf

1、中國協(xié)和醫(yī)科大學中國醫(yī)學科學院碩士研究生學位論文杏鮑菇液體發(fā)酵及多糖對小鼠脾淋巴細胞增殖作用的研究研究生：導師。學科專業(yè)：研究方向：入學時聞：所在院所：姚自奇蘭進研究員生藥學藥用真菌學皇OO二年九月藥周植物研究輯2005年5月北寒PekingUnionMedicalCollegeChineseAcademyofMedicalSciencesA8S羊RAC彳ABSTRACTPleurotuseryngiiisamedicinalanded

2、iblefungusMostofthestudieswerefocusedonthecultivationtechnologyandtherewerefewreportsontheliquidfermentation，effectivecomponenfsandpharmacologyrecentlyThemycelialantagonistictest，esteraseisozymezymogram，growthrate，biomas

3、sandpolysaccharidecontentof4differentPleurotuseryngiistrainswerestudiedinthispaperThe4strainshadgeneticdiversityThemycelialgrowthrateofthestrainX3wasthefastestbeingO69cmperdayThemycelialbiomassofthestrainX2wasthehighestw

4、ith1572：g／100mLThepolysaccharidecontentsofthestrainX1was3907pg／mgwhichwasthelowestHoweverthepolysaccharidecontentsofthestrainsX2、X4andXswere8428／tg／mg、7920pg／mgand7899肛g／mgrespectivelyThestrainX2wassuitableforliquidferme

5、ntationAnoptimummediumforthestrainX2liquidfermentationwasdeterminedbyorthogonalexperiment：409whitesugar309(NH4hS04，309yeastextract，509wheatbran，3gKH2P04，15gMgS04’7H20，1000mLwaterThesuitableconditionoffermentationforthest

6、rainX2wasdeterminedtOO：theinitialpH60，culturetimel44h，inoculationvolume10％，content(net)100mL(250mLconicalflask)ThebiomassandpolysaccharidecontentoftheliquidfermentationwerenoteffectedbythelightInaddition，thefermentatione

7、xperimentonoptimalmediaandcultureconditionwascarriedoutTheresultsshowedthatthepolysaccharidecontentsofmycelialandsubmergedfermentationliquidwere103timesand929timeshigherthantheunoptimizedThepolysaccharideofPleurotuseryng

8、ifcouldpromotethespleenlymphocyteproliferationinmiceinvitroby1mg／mLand02mg／mLTheresultsdemonstratedthatthepolysaccharideofPleurotuseryngiienhancedthemiceimmunologyKeywords：Pleurotuseryngii；Antagonistictest；Esteraseisozym