北方黃胡蜂和德國黃胡蜂（工蜂）腦中5-HT能神經(jīng)元系統(tǒng)免疫細胞化學定位研究.pdf

1、東北師范大學碩士學位論文北方黃胡蜂和德國黃胡蜂（工蜂）腦中5HT能神經(jīng)元系統(tǒng)免疫細胞化學定位研究姓名：李霞申請學位級別：碩士專業(yè)：動物學指導教師：暴學祥20050501AbstractApplyingColophonyPamffin(CP)tissueembeddingtechniquecombinedwithimmunocytochemicalStreptavidinPeroxidase(SP)method，wehadinvestig

2、atedthedistributionofserotonin(5HT)immunoreactiveneuronsystemintheprotocerebrumofVespulatufatufaandVespulagermanicaResultsshowedthatthebrainofwaspsisdeveloped，andtheprotocerebrumisthelargestinthebrain，almostoccupyingthre

3、efourthsofthewholebrainvolumeThebrainstructuresaresimilarinthetwowasps，andtheprotocerebrumiscomposedofseveralobviousstructuredneuropilsincludingthemushroombody(Ma)，centralcomplex(CC)，opticlobes(OL)，lateralaccessorylobe(L

4、AL)，andanterioroptictuberclerAOTu)MushroombodyispairedandsymmetricaldistributedOpticlobeisthevisioncenteroftheinsect，whichconsistsofthreeconcentriczones，thelamina，medull如andlobulaSerotoniniswidelydistributedthroughoutall

5、areasofthebrainThenumberofserotoninlikeimmunoreactivesomataintheprotocerebrumisabitsmall，andthepositiveneuronsaremainlysymmetricallylocalizeddorsalorposteriortotheprotocerebrumInadditionabout74and76serotoninpositivesomat

6、aclusteringinto8groups，arerespectivelyobservedintheprotocerebrumofVespulatufa，咖andVespulagermanicaInboththetwowasps，theserotoninimmunostainingintheanterioroptictubercleisweakerthanthatinthesurroundingneuropilsHoweveritis

7、muchstrongerinVespulatufarufathanthatinVespulagermanicaIntenseserotoninimmunoreactivitycanbefoundinthepedunclesofmushroombodies，thelateralaccessorylobes，thelateralneuropilsofprecerebralbridge，andthelargeregionofthecentra

8、lbodyanditssurroundingneuropilsMoreover，theserotoninimmunoreactivevaricosefibresformthickneuralnetworkinthecentralbodysurroundingneuropilsandthesuboesophagealganglion(SOG)Therearenotableserotoninpositivefibreprojectionsi

9、nthecalyces，opticlobes，andantennallobes，buttheKenyoncellsandthepostcerebralbridgearenegativelylabelledTheserotoninimmunoreactivityinthedorsallobes，tritocerebrum，andsuboemphagealganglionisrelativelystrongTheserotoninposit

10、iveneuronsinthesuboesophagealganglionsendmostprocessesintotheneuropil，wheretheyformafewthickfibretractsOnthewhole，noobviousdifferencesofserotonindistributionpatternaredetectedintheneuropilsbetweenthetwoinsectspecies，whil