兩種鞘翅目昆蟲細胞培養(yǎng)及昆蟲細胞系染色體分析.pdf

1、中圉掉譬蟹譬嘲憲穗學位論文兩種鞘翅目昆蟲細胞培養(yǎng)及昆蟲細胞系染色體分析張欣申請學位級別：碩士指導教師：墨筮盟寶員接睦嚏嗚盟窒亟盥論文完成實驗室：國窒鹽些魚資遺星童壁直魚歪』旦重：量塞墜窒論文答辯時間：2QQ魚生Z月④中國北京ABSTRACTTheCellCultureofTwoInsectsfromColeopteraandtheChromosomeAnalysisoflnsectCellLinesZhangXin(Biochemist

2、ryandMolecularBiology)DirectedbyProFengYing&ProChenXiaomingABSTRACTThetechniqueofinsectcellculture，asoneofthebasesofcellengineering，isavaluablemethodofmodernexperimentalbiologyItisappliedwidelytovariousfieldsofbiologymed

3、icineandagricultureDuetothesituationthatthereisnoanycelllinefromColeopterainsectshavebeenestablishedatpresentinChinadifferenttissuesoftwoColeopterainsectshavebeenculturedinthisstudyItprovidedthenecessarybasictheoryanduse

4、fulreferenceforsettingupthenewinsectcelllinesfromColeopteraAndthechromosomesofteninsectcelllinesalsohavebeenstudiedwhichprovidedimportanttheoryreferencefortheresearchofinsectcellsandenrichedthedataoftheinsectcellsbank1Ce

5、llculturefortwoinsectsfromColeopteraInthisresearch，differenttissuesofBlapsrhynchopteraandTenebriomolitorhavebeenculturedindifferentcultureconditions，andthesuitablecultureconditionsofeachtissuehavebeenfoundout：(1)Cellcult

6、urefortheneonatelarvaeofBlapsrhynchopter：Sterilizedthesurfaceoftheeggsby05％hibitanein75％ethanolsolutionfor5raincouldachievedthebeststerilizedeffectsanditwouldntpreventthehatchoftheeggsThemethodoftissuefragmentandmodified

7、Grace’SmediumweresuitableforthecellcultureoftheneonatelarvaeofBlapsrhynchopter(2)CellculturefortheembryosofBlapsrhynchopter：modifiedGrace’SmediumWasmoresuitableforitsculture(3)CellcultureforthehemocytesofBlapsrhynchopter

8、：Itcouldachievedthebeststerilizedeffectsandthemostanaountsofbloodbysterilizedtheadultinsectin75％ethanolfor8minandSchneidermediumWasfavorableforitsculture(4)CellcultureforthetestesofBlapsrhynchopter：Ithadnodifferentresult

9、sbythemethodoftissuefragmentorthemethodofsinglecell，andSchneidermediumwasfavorableforitsculture(5)CellculturefortheovariesofBlapsrhynchopter：nemethodofsinglecellWasbetterthanthemethodoftissuefragmentforitscultureandthere

10、sultsoffourmediumshevebeenusedinthecultureweresimilar(6)CellcultureforthefatbodiesofTenebriomolitor：Ithadnodifferentresultsbythemethodoftissuefragment01“themethodofsinglecell，andSchneidermediulnwasfavorableforitscultureA