酵母中coiled-coil domains的系統(tǒng)研究.pdf

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2、校址：甘肅省蘭州市蘭州大學(xué)碩士學(xué)位論文Systematicanalysisofcoiled—coildomainsinbuddingyeastAbstractEukaryoficproteinsarensuallyconstructedinacaste—likefashionusingdomainsandmotifsasbuildingblocksthatmediatespecificmolecularinteractionsAmong

3、suchdomains，thecoiledcoilhasbeenfrequentlyfoundinmediatingproteinproteininteractionsToanalyzetheinteractionspecificitybctxvecncoiledcoils，wedevelopedamulti—stepstrategythatcombinescomputationandexperimenttoidentifyspecif

4、iccoiledcoilinteractionsInstep1ailcoiledcoilswerepredictedfortheproteomeofsacchromysescerevisiaenlcproteinsthatcontaincoiledcoils戤denotedaseoiledeoilproteinsInstep2acoiled—coilproteininteractionnetworkWaSobtainedbyonlyco

5、nsideringinteractionsbctweencoiled七oilproteinsintheknownproteinproteininteractionsinyeastInstep3，acomputationalprogrambasedonSVMwasusedtopredictthepotentialcoiledcoilInteractionsInstep4。thepote】atialcoiledcoilpairsweresu

6、bjecttoayeasttwohybridassayIntotal，weanalyzedtheinteractionsfor4149coiledcoilpairs478ofthemarepositivesinvoIving202coiledcoildomainsFurthermore，weattemptedtoexplorethefunctionalroleofcoiledcoilsWhenacoilcdcoilthatiSinvol

7、vedinmethionemetabolicpathwaysWasknockedout，yeastexhibitedmethionineauxotrophindicatingtheessentialroleofcoiledcoilinsuchbiologicalpathwayMeanwhile，wedevelopnewtechniquestosystematicallydetectcoiledeoilinteractionsinvivo