家族性急性髓系白血病相關(guān)新基因全長cDNA的克隆及功能初步預(yù)測.pdf

1、V950228福建醫(yī)科大學碩士研究生畢業(yè)論文家族性急性髓系白血病相關(guān)新基因全長cDNA的克隆及功能初步預(yù)測CIOningandfunctionpredictionof恤eFuniengtbeDNAofanovelgeneidentifiedin“miIiaIacutemyelogenousleukemia所在院(系)t福建醫(yī)科大學協(xié)和臨床醫(yī)學院研兜生t王程教學科(專業(yè))t內(nèi)科學(血藏商學)導師t壬步元教授林旭敦授研兜起止日期t2004年

2、9月至2005年4月二oo六年四月CloningandfunctionpredictionoftheFulllengtheDNAofanovelgeneidentifiedinfamilialacutemyelogenousleukemiaAbstractIthasbeenprovedbyformerresearchesthatacuteleukemiaisatIlmourrelatingtomulti—genesCloninganov

3、elleukemiaassociatedgeneisonehotspotinthefielditnotonlyfacitiliatetoexplorethemechanismofmalignanthematopoieticdiseases，butalsotofindnewtargetsitesfortherapy[ObjectiveIToclonethefull—lengtheDNAofanovelgeneoffamilialacute

4、myelogenousleukemiaandtoexplainthemolecularmechanismsofthediseaseatthegenelevel[Method]ourlaboratoryconstructedasubtractiveeDNApooloffamilialacutemyelogenousleukemiausingthemethodofsuppressionsubtractivehybridization(SSH

5、)in2002andobtained28clones；28clonesweresequencedandcomparedwithknownsequencesinthepublicdatabasesofGenBank／EMBL／DDBJusingNCBIBLASTforhomologyanalysis，Thenfoundthat17sequenceswereapartofknowngenes，andtherestswereverifiedu

6、nknownfragments；detectingmRNAoftherest11unknownsequencesinfamilialacutemyelogenousleukemiapatiemsampleandhealthadultsamplewithonestepsemi—quantitativereversetranscriptase—PCR(RTPCRlinordertoexcludethepossibilityoffalsepo

7、sitivityfromSSH，4sequenceswereconfirmedtobepositivediflbrentiallyexpressedgenesfragmentsOnthebasisofthe4sequences，theexperimentwasdesignedasfollow：1clonethefulllen群heDNAofanovelassociatedgeneoffamilialacutemyelogenousleu

8、kemiabytheelectroniceDNAcloningandSMARTrapidamplificationofeDNAends(SMARTRACE)；2predicttheirassociatedfunctionbybioinformaticsstrategy[Results]Onesequenceof2257bpWasobtainedwithcompleteORFandpolyAtail，andlocalizedon4q311