BMI-1和P16INK4a在非小細胞肺癌中的表達及其臨床意義.pdf

1、蘇州大學碩士學位論文BMI1和P16INK4a在非小細胞肺癌中的表達及其臨床意義姓名：朱鋒鋒申請學位級別：碩士專業(yè)：胸心外科指導教師：高文20090501BMI1和P16INK4a在非小細胞肺癌中的表達及其臨床意義英文摘要ExpressionandClinicalSignificanceofBmi—1andP16INK4ainNSCLCAbstractObjectiveToinvestigatetheassociationbetween

2、theexpressionofBcell—specificMLVintegrationsite1(Bmi11andP16懈48inNSCLC(nosmallcelllungcancer)andtheirclinicopathologicsignificanceMethodsThesamplesofcanceroustissuesandparacanceroustissueswerecollectedfrom20patientswithN

3、SCLCTheexpressionsofBmi1andP16玳K48proteinweredeterminedbyWesternblotTheexpressionofBmi1andP16酣K48weredetectedbyEnVisionimmunohistochemistrymethodintissuemicroarraypreparedfrom130NSCLCpatientsStatisticalanalysisWasperform

4、edtoinvestigatetheassociationbetweentheclinicopathologicfeaturesandBmi1，P16帳婦expressionResults(1)TheresultsofWesternblotdemonstrstedthattheexpressionofBmi一1incanceroustissuesweresignificantlyhigherthanthatinparacancerous

5、tissuesandtheexpressionofP161NK48incanceroustissuesweresignificantlylowerthanthatinparacanceroustissues(2)TheresutsofimmunohistochemicalstudyalsoshowedthattheexpressionofBmi1andP16ⅣK48proteinwere8539％and4462％incanceroust

6、issuesrespectivelyONSCLCwithmoderateorstrongBmi1expressionweremorelikelytohavelowleveloftheP16聃K48protein(P=00001)FromourstudywefoundthattheexpressionofBmi一1wassignificantlyhigherinstageIll(P=O02)tumorscomparedtostagesIa

7、ndIItumors；@TheexpressionsofBmi一1proteinwerehighlycorrelatedwithlymphnodemetastasis(P=00008)，Histology(P=001)，TNMstage(P=002)andNstage(P=00073)：TheexpressionsofP16NK48proteinwerehighlycorrelatedwithlymphnodemetastasis(P=