殼聚糖及其衍生物對脂蛋白、瘦素及胰島素的作用研究.pdf

1、天津大學(xué)碩士學(xué)位論文殼聚糖及其衍生物對脂蛋白、瘦素及胰島素的作用研究姓名：熊熠燕申請學(xué)位級別：碩士專業(yè)：材料學(xué)指導(dǎo)教師：劉曉非20090501ABSTRACTInthispaper，twokindsofchitosanderivativesweresynthesizedbychemicalmodificationOcarboxymethylchitosan(OCMCs)and(2一hydroxyl一3dimethyllongchaina

2、mmonium)propylchitosan(NoCl6QCs)Thestructure，crystallabilityandelectricalchargecharacterswerecharacterizedbyFourierTransformInfraredSpectroscopy(FTIR)，XRayDiffractometer(XRD)andzeta(X)potentialrespectivelyToexplorethemec

3、hanismforchitosanandderivatives’abilitytoloseweightandlowerlipids，effectsonlipoprotein，leptinandinsulinwereobservedinobeseratsandinvitroMoreover，hepatocyteswerecultivatedtostudytheeffectoninterrelatedenzymesoflipidmetabo

4、lismbychitosanandderivativesResultsshowthatthedegreeofcrystallinityofOCMCsandNCi6QCsaredecreased‘significantlyasthechangingofthemolecularstructureinchitosan，andtheelectricalchargefortheNC16QCsishighlycationic(X=361my)，wh

5、ereasOCMCsisanionic(X=3182mV)ItiscertainlytestifiedthattwochitosanderivativeswerepereparedsuccessfullybychemicalmodificationResultsofanimalexperimentshowthatthetwochitosanderivativescouldsignificantlydecreasebloodglucose

6、contentandregulatebloodlipoproteincontentComparedtotheobesemodelgroup，thedecreaseofTCwasuptO1725％andtheconcentrationofLDLwas039士021mmol／Lindruggroup，closetonormallevelTheconcentrationofbloodglucosewasloweredtoo，whileHDLc

7、ontentwaschangedlialeThemaximumeffectofOCMCsandNC16QCsonthebloodleptinis5482％and6417％respectivelyandinsulincontentWaSraisedtoacertainextentInvitroassayshowthatthelargestadsorptionofTCandLDLwere3332％and189％，closetonormall

8、evelAndabsorptionofTGwasashighas2709％MoreovertheeffectofchitosananditsderivativesonleptinlevelWassignificantwiththelargestadsorption44。3％：Atthesametime，absorptionofinsulinwasalsosignificantasthegreatestadsorptionwas735％R

9、esultsofthehepatocyteexperimentshowthatchitosanandderivativestooklittleeffectontheexpressionofLDL＆LCATandHMGmRNA，whileincresedtheexpressionofHLmRNAsignificantlyKEYWORDSobesity，chitosanderivatives，lipoprotein，leptin，insul