體外菲立磁標(biāo)記mscs在3.0t磁共振儀下成像的研究

1、福建醫(yī)科大學(xué)碩士學(xué)位論文體外菲立磁標(biāo)記MSCs在3.0T磁共振儀下成像的研究姓名：朱榕城申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：@指導(dǎo)教師：譚建明@體外菲立磁標(biāo)記 MSCs 在 3.0T 磁共振儀下成像的研究 2Abstract [objective] To study the superparamagnetic iron oxide particles (superparamagnetic iron oxide particles, SPIO) la

2、beled SD in vitro rat bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) the feasibility of its SD rat bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) biological activity. Of superparamag

3、netic iron oxide particles (superparamagnetic iron oxide particles, SPIO) in vitro marker of SD rat bone marrow mesenchymal stem cell (mesenchymal stem cells, MSCs) in the cell under 3.0T Magnetic Resonance Imaging Trace

4、r feasibility.. [materials and methods] 1. Isolated MSCs from SD rat bone marrow, culture and identification of MSCs. 2.In vitro combined with different concentrations of poly-lysine tag SPIO, unlabeled cells as control

5、. Prussian blue staining and electron microscopy the cells were iron particles. 3.Trypan blue staining to detect cell survival. 4.Magnetic marker MSCs into adipogenic, osteogenic induction medium after culture were ide

6、ntified. 5.Application 3.0T Magnetic Resonance h2d1_154 sequences and tseBR2d1_16 sequences labeled cells on magnetic imaging. [Conclusions] 1. Prussian blue staining and electron microscopy showed dense cytoplasm contai

7、ning iron particles 2.Trypan blue staining showed that activity of magnetic markers on MSCs was no significant difference (P> 0.05) 3.labeled cells can be normal as fat, bone formation cell differentiation. 4.h2d1_154

8、 sequence and tseBR2d1_16 sequence tips and unlabeled cells in signal strength (SI) compared to, 1 × 106 (labeled cells), 5 × 105 (labeled cells) SI were significantly decreased (P 0.05). [Conclusion] Combined

9、 SPIO polylysine successful tag MSCs, magnetic markers and potential differentiation of cells had no effect. Magnetic labeled cells to generate the MR signal changes characteristic of the low. Application 3.0TMR imaging