湖泊浮游植物動態(tài)及微囊藻監(jiān)測技術(shù)研究——以淺水富營養(yǎng)化湖泊玄武湖為例.pdf

1、南京師范大學(xué)碩士學(xué)位論文湖泊浮游植物動態(tài)及微囊藻監(jiān)測技術(shù)研究——以淺水富營養(yǎng)化湖泊玄武湖為例姓名：徐瑤申請學(xué)位級別：碩士專業(yè)：環(huán)境地理學(xué)指導(dǎo)教師：王國祥20070514DynamicofPhytoplanktonandMonitoringofMicrocystisinaShallowEutrophicationLake，LakeXuanwuAbstractWiththeinereuslngdevelopmentofeconomyandh

2、umanactivities，environmentalproblemscausedbyeyanobactedalbloomsinvariouswatcrbodiesespeciallyinfreshwaterlakeshasbecomeaconc目／nallovertheworldXuanwulake，astatelevelscenicspotwiththetypicalsubtropicalclimate，whichlocatedi

3、nthemiddleandlowerreachersoftheyangtzeRiverformedeyanobaetedalbloominthesummerof2005andthedominantspecieswereMicrocystissppInthisstudybasedoRtheestablishmentoftheReal—timePC凡variationsofeyanobaeteriaMicrocystis，mieroeyst

4、inprodueingMicrocystiswegesurveyedRelationshipsbetweeathephytoplanktonandtheenvironmentalfactorswerealsostudiedinalongperiordThemainresultsandconclusionsWereasfollows：(1)17yearsmonitoringdatashowsthatwiththedecreasingoft

5、henutritiontherewasobviousfluctuationtothephytoplanktonbiomassSpeciesconstructionofphytoplanktonwaschangedformChlorellaCyelotella，Dacfylocoecopsis，MerismopediatoMicrocystiMerismopediaLowernutrientispronetothebreakoutofth

6、eeyanobaeteria(2)TomonitorthedensitiesofthepopulationofMicrocystis，CyanobacteriaandthepotentialmicroeystinproducingMicrocystisweusedaquantitativerealtimePCRassaytragefingthe16SCyan16SMicr‘mierocystinsynthetasegene(meyB)Q

7、uantificationbasedonrealtimePCRisapowerfultoolfortherapidquantificationofcyanobaeteriaMierocystis，MieroeystinproducingM／erocystisinenvironmentalsamples，espeseiallyitistoolowtobedetectedbymicroscopeThedetectiverateis100％a

8、ndthelowestdetectionis10cellsml～(3)Thedatashowsbelow：①TheabundanceofCyanobaaeriaceilsvariedfrom46x106to20xlO”cellsL1，theabundanceofMierocystiscellsvariedfrom44x105to90x109cellsL1，theratioofthemeyBsubpopniationtotheMicroc

9、ystisvariedconsiderablyfrom02％to11秭(螢Duringtheyearroundinvestigation：thereisnosignificantlycorrelationshipbctweellM／crocys出，MicrocystinproducingMbocysasandotherenvironmentalfactorsexceptTPP04ECyanobucteriahasthepositiver

10、elationshipwithTPP04PandpH，whilethenegativerelatioushipwithTN：TPandSD。@Duringtheinvestigationofwinterand洲I瑪theincreasingofMicro斜snsinlowerlayerismorefastthaninmiddleandtoplayersinthewatercolonyTheMicrocystiswerecloselyco