中國(guó)藥科大學(xué)中藥學(xué)專業(yè)英語(yǔ)課件--丹參

1、丹 參,Radix Salviae Miltiorrhizae,2,Main Contents,OriginsPlant MorphologyCollection and PreparationProduction and CommerceDescriptionMicroscopical CharactersChemical ConstituentsPhysical and Chemical Identification

2、High Performance Liquid Chromatography,3,Plant Morphology,A perennial herb, densely pubescent and glandular hairy throughout. Root cylindrical, vermillion. Stem 4-angled, branched at the upper part. Leaves opposite, pin

3、nately compound; leaflets 5～7, ovate or elliptical-ovate, serrate along the margin, pubescent on both surfaces.,vermillion：朱紅色 angled：棱 serrate：鋸齒狀的,4,Flowers in terminal or axillary verticillate pseudo-racemes; ca

4、lyx bell-shaped; corolla bluish-purple. The upper lip erect, slightly falcate, retuse at apex, the lower shorter, 3-lobed, the middle lobe larger, 2-cleft; stamens 2, filaments short, connectives long, the upper end of t

5、he connective bearing a fertile anther-cell, the lower end with an imperfect one; ovary deeply 4-lobed, style gynobasic. Nutlets 4, ellipsoid.,verticillate：輪生 angled：棱 pseudo-：假的 retuse: 凹形falcate: 鉤狀的 filame

6、nt: 花絲 gynobasic：花柱,5,Description,Root cylindrical or conical, usually curved, branched,10～30 cm long, 0.3～1.5 cm in diameter. Externally brick-red, brownish-red or purplish-brown, rough, longitudinally furrowed or wri

7、nkled, the bark of old roots mostly scaling off, sometimes crowned with remains of stem base. Texture hard and fragile, fracture fibrous, bark dark reddish-brown to purplish-brown, wood showing bundles of vessels, yellow

8、ish-white, arranged radially. Odour, slight; taste slightly astringent.,6,Transverse section,Cork consisting of several layers of cells, mostly containing orange or purplish-brown contents. Phelloderm narrow. Phloem rela

9、tively broad. Cambium ring distinct. Xylem bundles radial, consisting of vessels, xylem fibres and xylem parenchyma. Xylem fibres often accompanied by vessels. Stone cells scattered in phelloderm and phloem.,cortex: 表皮

10、 phelloderm: 栓內(nèi)層,7,Reddish-brown. Stone cells occurring singly and scattered throughout, subrounded, subrectangular, subfusiform or irregular, up to 257 μm long, 20～65μm in diameter, some of them containing brown conte

11、nts. Reticulated and bordered pitted vessels, and bundles of xylem fibres frequent. Cork cells yellowish-brown, subsquare or polygonal in surface view, containing reddish-brown pigments, dissolved on mounting in chloral

12、hydrate solution.,Powder,surface view: 表面觀 mount: 做標(biāo)本 chloral hydrate: 水合氯醛,8,TanshinoneⅠ,tanshinone ⅡA, tashinoneⅡB, cryptotanshinone, dihydroisotanshinone Ⅰ, hydroxytanshinone, isotanshinone Ⅰ,Ⅱ, iso- cryptotanshinon

13、e, miltirone, salviol, etc.【化學(xué)成分】 丹參酮Ⅰ，丹參酮ⅡA，丹參酮ⅡB，隱丹參酮，二氫丹參酮Ⅰ，羥基丹參酮，異丹參酮Ⅰ、Ⅱ，異隱丹參酮，丹參新酮，鼠尾草酚，等。,Chemical Constituents,9,（1）Boil 5 g of the powder in 50 ml of water for 15～20 minutes, cool and filter. Concentrate th

14、e filtrate on a water bath, dissolve the extract in 3～5 ml of ethanol, filter. Apply several drops of the filtrate to a piece of filter paper, allow it to dry and examine under ultra-violet light (365nm) , a bright bluis

15、h-grey fluorescence is produced. Expose the filter paper to ammonia vapour for 20 minutes, remove the filter paper and examine again under ultra-violet light (365 nm ), a pale bluish-green fluorescence is produced.,Physi

16、cal and chemical Identification,10,【理化鑒定】,（1）置5g粉末于50ml水中煎煮15~20分鐘，冷卻后過(guò)濾.在水浴中濃縮濾液，用3~5ml乙醇溶解濃縮產(chǎn)物，過(guò)濾。將濾液在濾紙上點(diǎn)一些斑點(diǎn)，斑點(diǎn)揮干后在365nm波長(zhǎng)紫外光下觀察，可看到明亮藍(lán)灰色熒光。將濾紙置于氨氣中20分鐘后，在365nm波長(zhǎng)紫外光下觀察，可見淡藍(lán)綠色熒光。,11,,（2）To 0.5 ml of the filtrate obt

17、ained from Identification (1) , add 1～2 drops of ferric chloride TS: a dull green color is produced.,ferric chloride:三氯化鐵 TS：test solutiondull: 暗的 pale: 淺的，淡的,（2）從（1）的濾液中移取0.5ml，加1~2滴氯化鐵TS：可見濾液變?yōu)榘稻G色。,12,(3) To 1

18、 g of the powder add 5 ml of ether, shake in a stoppered test tube and stand for 1 hour and filter. Evaporate the filtrate to dryness. Dissolve the residue in 1 ml of ethyl acetate as the test solution. Prepare a solutio

19、n of Radix Salviae Miltiorrhizae reference drug in the same manner, used as the reference drug solution. Dissolve Tanshinone ⅡA CRS in ethyl acetate to produce a solution of 2 mg per ml as the reference solution. Carry o

20、ut the method for thin layer chromatography, using silica gel G as the coating substance and benzene-ethyl acetate(19:1) as the mobile phase.,a stoppered test tube: 具塞試管 stand for ：靜置 residue: 殘?jiān)?reference dr

21、ug:對(duì)照藥材 CRS：Chemical Reference Standard,13,Apply separately to the plate 5μl of each of the three solutions. After developing and removal of the plate, dry it in air. The spots in the chromatogram obtained with the

22、 test solution correspond in position and colour to the spots in the chromatogram obtained with the reference drug solution; and the dark-red spot in the chromatogram obtained with the test solution corresponds in positi

23、on and colour to the spot in the chromatogram obtained with the reference solution.,corresponds to: 對(duì)應(yīng)于，與 一致,14,,（3）取1g粉末加5ml石油醚于有塞試管中振搖，靜置1小時(shí)后過(guò)濾。將濾液蒸干。殘?jiān)?ml乙酸乙酯溶解后作為供試品溶液。用同樣方法制備丹參對(duì)照藥材的溶液，作為對(duì)照藥材溶液。將丹參酮ⅡA CRS溶解在乙酸乙

24、酯中制成每ml中含2g丹參酮ⅡA溶液，作為對(duì)照品溶液。照薄層色譜方法，用硅膠G作為固定相，苯-乙酸乙酯（19：1）作為展開劑。將3份溶液分別點(diǎn)5μl于薄層板上，展開分離后在空氣中晾干。供試品溶液的色譜中在對(duì)照藥材溶液色譜的相應(yīng)位置上有相同斑點(diǎn)；并且供試品溶液色譜中的深紅色斑點(diǎn)在與對(duì)照品溶液色譜相應(yīng)位置上有相同斑點(diǎn)。,15,Pharmacology,(1) Coronary artery-dilating effect : It can

25、increase blood flow by dilating coronary arteries.(2) Sedative effect (3) Antibacterial and antifungal effects: It strongly inhibits Staphylococcus aureus, and has a definite inhibitory action on Mycobacterium tubercul

26、osis and some dermatophytes.【藥理作用】 （1）擴(kuò)張冠狀動(dòng)脈作用：通過(guò)擴(kuò)張冠狀動(dòng)脈可增加血流量。（2）鎮(zhèn)靜作用（3）抗菌殺菌作用：能強(qiáng)烈抑制金黃色葡萄球菌，并能明顯抑制肺結(jié)核桿菌和一些皮膚真菌。,16,Action,To promote blood circulation and stimulate menstrual discharge, to remove blood stasis and r

27、elieve pain, and to nourish the heart and tranquilize the mild.【功效】 活血調(diào)經(jīng)，祛瘀止痛，養(yǎng)心安神,17,Indication,Menstrual disorders, amenorrhea, dysmenorrheal, postpartum abdominal pain due to blood stasis, neurasthenia, angina pect

28、oris in coronary heart disease, hepatosplenomegaly, carbuncles and erysipelas.【適應(yīng)癥】 月經(jīng)不調(diào)，閉經(jīng)，痛經(jīng)，產(chǎn)后血液停滯引起的腹痛，神經(jīng)衰弱，冠心病心絞痛，肝脾大，癰和丹毒。,18,Tanshinone IIA Carry out the method for High Performance Liquid Chromatography (Appe

29、ndix VI D).Chromatographic system and system suitability Use octadectylsilane bonded silica gel as the stationary phase and a mixture of methanol and water (75:25) as the mobile phase. As detector a spectrophotom

30、eter set at 270nm. The number of theoretical plates of the colum is not less than 2000, calculated with the reference to the peak of Tanshinone IIA.Reference solution Accurately weigh 10mg of tanshinone IIA CRS to

31、a 50ml amber volumetric flask. Dissolve and dilute with methanol to volume and mix well. Accurately measure 2ml to a 25ml amber volumetric flask, dilute with methanol to volume and mix well (containing 16mg of tanshinone

32、 IIA per ml).,Assay,19,Test solution Wigh accurately 0.3g of the powder (through a No.3 sieve) to a stopper conical flask. Add accurately 50ml of methanol, and weigh. Heat under reflux for 1hour, cool, weigh again,

33、make up the loss of weight with methanol, mix well and filter , use the successive filtrate as the test solution.Procedures Accurately inject 5ml of each of the reference solution and the test solution, respectivel