21354.準(zhǔn)噶爾雅羅魚的核型和帶型研究

1、石河子大學(xué)碩士學(xué)位論文準(zhǔn)噶爾雅羅魚的核型和帶型研究姓名：王佳君申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：胡文革201106IIIAbstractLeuciscusmerzbacheribelongstoCyprinifmes，Cyprinidae，Leuciscinae，Leuciscuscuvier，alsoknownasXinjiangLeuciscus.Inthedivisionofthewldgeographical

2、distributionLeuciscusmerzbacheriistheuniqueindigenousfishinXinjiangonlyfoundintheJunggarBasinofXinjiangTianshannthernriversystems.Thefoodvalueofthefishhashighnutritionwhichfmerlywasthemaineconomicalfishinproductionareash

3、ashugeprospectsfartificialbreeding.SincemanyyearsbecauseofvariousreasonsthenatrualresourcesofLeuciscusmerzbacherihasbeendecliningitsextantnumberisalreadyextremelyscarcefacedwithendangeredconditionTherefeithastheimptantsc

4、ientificresearchvalue.Inthispaper，thestudyongeicdiversityofthekaryotypesbingpatternsofthemhasnotbeenreptedthusfar.BasedonthesecurrentstatusthechromosomekaryotypebingpatternsofLeuciscusmerzbacheriwerestudiedcomparedwhichw

5、erecollectedfromJingheKuitunregions.InthisstudythechromosomekaryotypebingpatternsofLeuciscusmerzbacheriwhichwerecollectedfromJingheKuitunregionswerecomparedbykaryotypetechniqueAgNs，CBGBsoastoanalyzethevariationonthelevel

6、ofcytogey（chromosome），WepreliminarilyanalyzedthechromosomediversityofL.merzbacheri.ThechromosomespecimensofLeuciscusmerzbacheriwereobtainedfrommetaphaseofheadkidneykidneycellsbycolchicinelowconcentrationairdryingtechniqu

7、ethenstudiedtheirGiemsastainAgNs，CBGBwerestudiedinthispaperThemainresultsofthestudywereasfollows:1L.merzbacherifromJingheKuitunhadadiploidchromosomenumberof2n=50itskaryotypefmulawas18m14sm6st12tNF=82sexchromosomewasnotfo

8、und.ThepossibleevolutionaryrelationsinchromosomewerediscussedamongLeuciscusmerzbacheriLeuciscusbaicalensisLeuciscusidus2TheAgNspolymphismsofL.merzbacherifromJinghewereindividuallyspecific1~2ofthenumberthefrequencyof1AgNs

9、wasthelowest(10%)whilethefrequencyof2AgNswasthehighest(70%).ThemainAgNspolymphismswerelocatedintheshtarmsofthechromosomem1m4.TheAgNspolymphismsofL.merzbacherifromKuitunwereindividuallyspecific1~2ofthenumberthefrequencyof

10、1AgNswasthehighest(68%)whilethefrequencyof2AgNswasthelowestt(25%).ThemainAgNspolymphismswaslocatedintheshtarmsofthechromosomem3.TheassociationsofAgNswerenotobserved.Thepossibleevolutionaryrelationsinchromosomewerediscuss

11、ed，theresonsofthedifferencewerealsodiscussed.Theresultsshowed:L.merzbacherifromJinghehadahigherevolutionarydegree3ThereweresomedifferencesintheCbingpatternsamongpopulationsinJingheKuitunwhichincludedthedifferentlocationn

12、umberofCb.Therewere1~4CbsonthechromosomesofL.merzbacheri2fthemostones.TheCbingpatternwasmainlyconsistedofcentromericbtelemtricbintercalaryb.Theresultsshowed:L.merzbacherifromJinghehadahigherevolutionarydegree，whichwasacc