提高奶牛性別鑒定胚胎移植妊娠率的研究.pdf

1、簿Y933632_蒸?！眗i疊一j媧妒囂蓬t遵碩__lj學(xué)位(畢業(yè))論文提高奶牛性別鑒定胚胎移植妊娠率的研究學(xué)位申請人：指導(dǎo)教師：學(xué)科專業(yè)：學(xué)位類別：授予單位：答辯日期：孫曉琳李樹靜教授田樹軍教授動物遺傳育種與繁殖農(nóng)學(xué)碩士河北農(nóng)業(yè)大學(xué)二oo六年六月十三日SthdyonimprovementofpregnancyrateofbovinesexedembryotransferPostgmduate：Sunxia01inM句or：Animal

2、Gene“cs，Breeding，andReproductionscienc。Adviser：“s11ujingTianshujunAbstractTheaimofthisscLldyistoesIablishaneffectivesvs把mmaImforindustrializaCioninchinaafterfactorsa玎ecttingpre印ancyratewereanalyzedtoilI】provepregIlancyfa

3、le800hi曲qualityda時cowsweresupemⅦlatedtoproduce鋤bryosinMuDanjiangLongdaLtdCoofHeiLon畫iangpmvinceaIldxinjiangJinniuGmupFemale廿nbryos咖sf打rcdoraRerAnalyzedpregnancyrateofsexed啪咖on∞lbryocharacte‘recipientcharacterand出eircon℃1

4、aIivityTheresultasfollows：11nt11easpectofembryoofthisexp“ment，bo血p心gIlancyrateofnonsexedandsexedembryoswere10wer髂thestagewashigheronnonsexedembryo，therewasnosiglli矗cantdiff拍nce伽pregnancyraleofmonlla—ealyblas【ocysIandblas

5、tocyst，blastocysandexpandedblastocysI(P005)，butsigni6cantatmomlaealyblasIocystandcxpandedblaslocyst(PO05)Pregnancyrateongra如1washi曲盯也angrade2ofsexedembryo2Theoperationofsexingtoembryoaf鳧ctpregnancyrateofscxed鋤bryoRegular

6、andsexedcHlbryoswerecomparedbetwe曲行cshandf而zen啪bryo，theresullindicatedthatmepregnancyrateofregular嘲bryotendtohighert11ansexingembryoof療eshorf如z肌embryo，butCherewasnosigni6canIdiff柏lce(P005)Compared講egnancyrateonfk8handf而z

7、enembryosofregularembryos，t11erewasnosign墑c柚tdif艷rence(PO05)，butverysignincantonsexedembryos(PO05)Therewasnodif毪renceondevelopInentalmteoutsideinvitrobemeene1Tlbryos誦min3hand3—5hfromnushingto船ezing(Po05)，butbomofthemwere

8、obViouslyhi曲ermanmoseel】小ryosfromnushingto療cezingmoretllall5h(P005)4Embryoswerec011ected舶mmedonors撕er75d土5hines廿us，meredultisjndicated血atallstagesofembyoscouldgelacc印tablepre印ancyratewhenlheywerennsf咖edtolherecipientsof7