10766.海洋沉積物中脂類生物標(biāo)志物的分析方法研究

1、分類號：分類號：P67P67密級：密級：不保密UDCUDC：504504學(xué)校代碼：學(xué)校代碼：1106511065碩士學(xué)位論文海洋沉積物中脂類生物標(biāo)志物的海洋沉積物中脂類生物標(biāo)志物的分析方法研究分析方法研究李小琳指導(dǎo)教師張培玉學(xué)科專業(yè)名稱環(huán)境科學(xué)與工程論文答辯日期2015年6月10日AbstractLipidbiomarkerscanprovidetheimptantreferenceinfmationaboutthesourceofga

2、nicmatterdistributiondepositionlawinmarinesedimentsaswellastheancientmarineenvironment.Thepaperdescribedthedeterminationofnalkanesfattyalcoholsfattyacidsinmarinesedimentsbygaschromatographymassspectrometrywithaccelerated

3、solventextraction(ASE)fsamplepretreatment.ThemainfactswhichaffecttheextractionyieldofnalkanesfattyalcoholsfattyacidsbyASEsuchastheproptionofextractantthetemperaturestatictimecycletimeswerediscussed.Thefollowingconclusion

4、sfromthestudy:whenthelongcarbonchainnalkanestobedetectedbehigherinsampleswerecommendusingmethanolmethylenechlide(V:V1:3)astheextractionsolventwhiletheshtcarbonchainnalkanestobedetectedbehigherinsampleswerecommendusingmet

5、hanolmethylenechlide(V:V1:9)astheextractionsolventthequantityofshtlongcarbonchainnalkanesthetotalnalkanesgraduallyincreaseswiththeincreasingofthetemperaturestatictimenumberofcycles.Theoptimumparametersfnalkanesextraction

6、frommarinesedimentswere:temperatureof150℃statictimeof15min3cycles.Theprecisionofmethodwas3%to14%RSD.Alternativesrecoverywas84114%.Theoptimumconditionsoffattyalcohol:thequantityofshtlongcarbonstraightchainalcoholsthetotal

7、straightchainalcoholsgraduallyincreaseswiththeincreasingofthetemperaturestatictimenumberofcycles.Theoptimumparametersfstraightchainalcoholsextractionfrommarinesedimentsare:theextractantofmethanolmethylenechlide(V:V1:3)te

8、mperatureof150℃statictimeof15min3cycles.Theprecisionofmethodisfrom3%to17%(n=5)therecoveryis77.5%to117.1%.Theoptimumconditionsoffattyacids:Ftheconditionsoftheextractiontemperaturestaticextractiontimedifferenttypesoffattya

9、cidsvaryregularlypresented.Themethodtoextractthefattyacidstomaximize(highestextractionefficiency)asthebasistooptimizetheoptimalexperimentalconditions:temperatureof110℃statictimeof10min3cycles.Theprecisionofmethodwas3%to2

10、3%RSD.Thismethodissimplefastefficienthavinghighrecoverygoodprecisionwhichisapplicabletothequantitativeanalysisofstraightchainalcoholsinmarinesediment.Westudiedthemethodsofseparationpurificationofnalkanesaromatichydrocarb