乙醇發(fā)酵產(chǎn)氫新菌株r12的分離鑒定及其產(chǎn)氫能效

1、圖書分類號：X7035UDC：62816理學碩士學位論文Y821209乙醇發(fā)酵產(chǎn)氫新菌株R12的分離鑒定及其產(chǎn)氫能效碩士研究生：導師：申請學位：學科、專業(yè)：所在單位：答辯日期：授予學位單位：程瑤任南琪教授理學碩士微生物學市政環(huán)境工程學院2005年12月哈爾濱工業(yè)大學哈爾濱工業(yè)大學理學碩士學位論文AbstractBiotechnologyofhydrogenproductionbyfermentation(BHPF)hasbecomeon

2、eofthebio—hydrogen—producingresearchhotspotsThestudyofscreeningthehighefficiencyhydrogen—procuctionbacteria(HPB)takesveryimportantroleonaccelerationtheindustrializationofBHPFelucidatingthehydrogenproductionmechanismofeth

3、anolfermentationpathwayandestablishmentofethanol—producingnewgenuscharacteristicsinprokaryotegroupsWiththeHungateanaerobicoperationtechniqueandbythewayofoptimizedscreening，ahighefficiencyHPB，strainR12fromtheethanoltypefe

4、rmentationactivatedsludgeinthecontinuous—flowbio—hydrogenproductionreactor(CSTR)，wasachieved、vimmaximumspecificH2producingrate(Q眥)of2611mmolH2／g’dry‘cell‘h。Identificationbasedonthemorphologyanalysisofphysio—biochemicalch

5、aracteristicsand16SrDNAgenesequenceindicatedthatthestrainR12WaSanovelspeiciesFttrthrnore，theethanoltypefermentationcommunityandthereasonablebacterialethanolfermentationpathwaywerepresentedWehavediscussedtheecologicalfact

6、orthatcallaffectthehydrogen—producingabilityofR12At34“CandinitialpH45，cellgrowthandhydrogenproductionofR12isthehighestSuitableconcentrationofFe2，M92，Ni2couldacceleratehydrogen—producingrateHoweverproductsinterminalhadneg

7、ativefeedbackonthebacterialpopulationThemostsuitablecarbonsolnccisglucose，anditissuitableforcellgrowthandhydrogenproductionat159／LofglucoseTheoptimalaugmentationstrategyandeffectofstrainR12wasinvestigatedduffngstablestat

8、eofCSTRStrainR12VCasaddedtocontinuousflowbio—hydrogen—productionreactorwithvariousVSSproportionTheresultsindicatedthattheaveragebiogasproductioncapacityandtheaveragehydrogenproductioncapacitywereimprovedwhencontinuous—fl