華山松高結實無性系選擇與無性繁殖技術研究.pdf

1、西南林學院2005屆碩士研究生學位論文華山松高結實無性系選擇與無性繁殖技術研究StudyonSelectionandAsexualpropagationTechniquesofHighFertilityPinusarmandiiFranchClones張興指導教師：李桐森教授西南林學院段安安教授西南林學院學位授予單位：西南林學院云南昆明Abstract：Withmorecognitionabouthighnutritioncontent

2、andsanitarianfunctionofthekernelsofPinusarmandiibeingknown，moreandmoreseedswillbedemandedHowevermostoftheexistingPingsarmandiiforesthavinglowreproductivecapacitySOit’SdifficulttosupplyenoughseedsItisnecessarytosearchaway

3、assoonaspossibletosupplylargequantitiesofseedlingspossessinghighfertilityviaselectingfertileclonesfromtheprimaryseedorchardSeedselectionhasbeenexploredpreliminarilyatZixishanclonalseed—orchardinChuxiongcountyYunnanprovin

4、ceforhighfertilityclones，aswellaspropagatingthroughcuttingandembryocultureIntheseedselection，thirteenhighfertihtycloneshavebeenselectedprimarilybyphenotype，alsothegeneralappraisalofthemhavebeenmadeviaavailableseedsquanti

5、tysingletreeandseedsyieldssingletreeEffectofcuttingageandkindsandcongcentrationsofplantgrowthregulators，culturemediaandclonesonrootingwasstudiedbycuttingInthispapereffectofdifferentplantgrowthregulatorscombinationonadven

6、titiousbudsdifferentiation，androotingalsoWasstudiedthroughembryocultureAndtheresultswereasfollows：①CloneNo44，42and56havekeptsuccessivelyhighyieldofconeintwoyears，andtheaveragenumbersofsingletreewereinfrontItindicatedthat

7、thesecloneshadhighfertility②Beingappraisedandselectedbyessentialseedsquantityofsingletreeandseedsyieldsofsingletree，cloneNo44，42，98and43excelledotherclonesinseedsyieldsofsingletree，andcloneNo44，84and25exceedothersinaspec

8、tsofsupplyingmoreavailableseedsW1lilecloneNo44，84。25，43and42hadpreferablecharacteroftheavailableseedsquantityofsingletreeandseedsyieldsofsingletree⑧Sandisthemostproperculturemediumforrootingamongvermiculite，forestsoil，an

9、dsand；AndIAAisthemostproperplantgrowthregulators麗mtheconcentrationat100ppmamongusedplantgrowthregulators④ThecuttingstudyofthethirteenclonesshowedthattherewasremarkabledifferenceofrootingrateamongclonesCoefficientofextend

10、ingpropagationwasthemostimportantfactorwhicheffectedthecapabilityofpropagation，andcloneNo59，8，42，101，25，84and44andwerebetter⑤Highconcentrationofthesugarwasbadtostartupculture；theculturemediumsupplementwithBAl5m礦5％sugarNA