大豆品質(zhì)性狀作圖群體的構(gòu)建及種子蛋白和脂肪含量QTLs的SSR標記分析.pdf

1、四川農(nóng)業(yè)大學碩士學位論文大豆品質(zhì)性狀作圖群體的構(gòu)建及種子蛋白和脂肪含量QTLs的SSR標記分析姓名：王曉丹申請學位級別：碩士專業(yè)：果樹學指導教師：李能芳朱保葛20050601ConstructionofamappingpopulationwithqualitytraitsandanalysisofSSRmarkerslinkedtoQTLsforseedproteinandoilcontentinsoybeanAbstractSoybe

2、anseedscontainabundantproteinandoil，whichisakindofimportantplantoilandthemajorsourceofplantproteinwithhighqualityAtpresent，theSOybeanvarietywithhighproteinorhighoilisabsentinourcountrySoitisanurgenttasktoimprovetheconten

3、tofsoybeanoilandproteinusingmodernbiologyandbreedingtechnologyBecausethesetwotraitsarethequantitativetraitscontrolledbymanygenes／loci，itisusefulforQTLmappinginthewayofapplicationInthisstudyaF2segregatingpopulationwascons

4、tructed，andseveralSSRmarkersassociatedwithlociforseedproteinandoilcontentwerescreenedusingthispopulationinordertOimprovesoybeanqualitybyusingMarkerassistedselectionThemainresultsofthestudyweresummarizedasfollows：1AF2popu

5、lationwiththedouble—qualitytraitswasdevelopedfromaCROSSbetweenKFl4and$8510Thispopulationcontained287plantsKFl4seedshavehigherproteinandloweroilcontent，whereasseedsof$8510havehigheroilandlowerproteincontentTheoilandprotei

6、ncontentofseedsofF2segregatingpopulationallappearedagreatsegregationthatexceededtheirparentsTheyallaccordedwiththenoHnaldistribution2Amongatotalof433pairsofSSRprimersusedinthisexperiment，109primerpairsweredetectedtOownpo

7、lymnrphismsbetweengenomesofparents，KFl4and$8510Thefrequencyofpolymorphismswas25％Among109primerpairs，6pairs，Satt331、Satt551、Satt572、Satt290、Satt202andSatt323，werepolymorphicbetweentwoprotein—pools，4pairs，Satt472、Satt345、S