對優(yōu)質(zhì)亞基、抗白粉病小麥的分子標記輔助選擇方法研究.pdf

1、貴州大學碩士學位論文對優(yōu)質(zhì)亞基、抗白粉病小麥的分子標記輔助選擇方法研究姓名：丁曉娟申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：楊昌河20060501貴州大學2006屆碩士學位論文TheStudyforMolecularMarkerassistedSelectionMethodinWheatofGoodSubunitandPowderyMildewRisistanceGeneAbstractThegenepyramidinginthem

2、aterialthatcontainedPm2landthematerialthatcontainedsubunitpair510weredetermined，andtheSCARbasedmarkerslinkingwithpowderymildewresistancegeneandthespecialPCRprimerscontrollingthegenesequenceof1Dyl0and1Dx5wereusedformolecu

3、larassistedselectioninhybridoffspringThemainresultsweresummarizedasfollowed：1Theresultof1Dx5and1Dyl0specificPCRmarkerswasaccordedwi也theresultofSDSPAGEinsomematerials，alsotherepetitionandspecialityofPCRresultswelegoodItin

4、dicatedthatusingthe1Dx5and1DylOspecialPCRprimertoselectsubunitpair510wasnotonlyfeasible，andaccuratelyreliable，anditwaseffectivetoselectthegoodqualityinwheatandprovidedthereliablebasisonMASofwheat2n玲MASefficiencyofSCARmOi

5、nBCIFlbetweenGuinon92landNeepawareached95_3％nleMASefficiencyofSCARl400inF3betweenJYJY970012andAi2002reached941％Thetwoefficiencydiffered12％11他resultshowedthattheSCARt400primerlinkedtoPm2lmorecloselythenthismarkercouldbeco

6、nvenientlyusedformarkerassistedselectioninwheatbreedingprogramsfortheidentificationorpyramidingofPr021，andshortenthebreedingprocess3Inthepopulationof100plantsF3inGuinon921xNeepawaand50plantsBCIFlinJYJY970012Ai2002couldse

7、lectthedmrentsubunitcombinationofplantswithresistedpowderymildewgenebythespecialPCRpriraersThusbywayofMAS，itcouldappraisethepurposegeneinearlyfilialgenerationandprovidethefIlrthermaterialofseedselectionforthewheatbreedin

8、gofgoodsubunitandresistantedgene，thusraisingtheselectionefficiency4HavingtheaidofthePCRmarkerthatlinkedtotheresistantedandqualitygenetoestablishMASSystemofgoodsubunitandresismntedwheatbreedingitcouldtaketheplaceofMAStoas