孕酮調(diào)節(jié)不明原因復(fù)發(fā)性流產(chǎn)患者淋巴細(xì)胞產(chǎn)生IL-4、IL-12與PPARγ的相關(guān)性研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文孕酮調(diào)節(jié)不明原因復(fù)發(fā)性流產(chǎn)患者淋巴細(xì)胞產(chǎn)生IL4、IL12與PPARγ的相關(guān)性研究姓名：廖義鴻申請學(xué)位級別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：李紅發(fā)20090501華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文3ThecrelationbetweenprogesteronemodulatingIL4、IL12cytokineoflymphocytefromwomenwithunexplainedrecurrentab

2、tionpparγPostgraduate:LiaoYihongSupervis:Prof.LiHongfaDepartmentofObstetricsGynecologyUnionHospitalTongjiMedicalCollegeHuazhongUniversityofScienceTechnologyWuhan430022ChinaAbstractObjective:Toinvestigatetheroleofprogeste

3、roneinmodulatingIL4、IL12cytokineproductionoflymphocytesfromwomenwithunexplainedrecurrentspontaneousabtion(URSA)findcrelationbetweenthisrolePPARγstudytheroleofprogesteroneinmodulatingTh1Th2balancethepossiblemechanism.Meth

4、ods:VenousbloodwasdrawedfromwomenwithURSAperipheralbloodmononuclearcells（PBMC）wereseparatedbydensitygradientcentrifugation.ThensuspendedPBMCinRPMImediumcontaining10%calfserumatadensityof106ml.Thisexperimentisdividedintot

5、wogroups:theprogesteronegroupthecontrolgroup.Thefmerwasculturedunder37℃、5%CO2withprogesteroneataconcentrationof105molLphytohemagglutinin(PHA)5ugmlthelatterwasculturedatalmostthesameconditionbutwithoutprogesterone.After72

6、hours’culturinginterleukin(IL)4、IL12intheCulturesupernatantsweremeasuredbyELISAwhiletheremainedcellswereextractedfthewholeproteintomeasuretheexpressionofPPARγbyWesternBlotting.Results:expressionofIL12intheprogesteronegro