玉米DH群體的遺傳分析及QTL定位初探.pdf

1、河北農(nóng)業(yè)大學碩士學位論文玉米DH群體的遺傳分析及QTL定位初探姓名：宋煒申請學位級別：碩士專業(yè)：作物遺傳育種學指導教師：劉志增20050601GeneticAnalysisofMaizeDoubledHaploidPopulationandPrimaryStudyonQTLsMajorCropGeneticsandBreedingPostgraduateSongWeiTutorLiuZhizengAbstractWithtwomaize

2、haploidpopulationsandoneDHpopulation，15agronomictraitsofDHpopulationwereanalyzedhereditarySegregationofthreepopulationsweredetectedbySSRGeneticmapofDHpopulationwasconstructedQTLswereidentifiedforsometraitsofDHpopulationT

3、heresultsweresummarizedasfollows：1WithmaizeDHpopulationderivedfrom“NongDanNo5”(Nongxi531Nongxi110)inducedbyparthenogeneticinducing—line，theheritabilityanalysisof15quantitationtraits，ieearlength，earperimeterearweJ“ght，cob

4、perimetercobweie#t，100lemelweight，rOWNo，kernel／rowrareearlength，planthei曲t，stemperimeterLUBLUN，numberoflateralbranch，daysfromsowingtoblooming，weremadeThenumberofgenepairscontrollingthesetraitswasestimatedBasedonkourtosis

5、andskewnessvaluesofDHpopulation，thegeneinteractioninthesetraitswasdiscussedTheresultsshowedthat，complementaryinteractionwasdetectedforrareearlength，daysfromsowingtoblooming，rowNoandcobwei曲tWhilethegenieinteractionwasfoun

6、dabsentforothertraitsItwassuggestedthat，earlength，earperimeter100lemelweJ【glltandplantheightareselectedinearlygenerationsbecauseofhi曲heritabilityfewgenepairsandabsenceofgeneinteraction2SSRmakersweredetected，withtwomaizeh

7、aploidpopulations‘DenghaiNo9”(872365232)and(Nongxi531K36)，andDHpopulation(con—taining72lines)derivedfrom“NongDanNo5”(Nongxi531Nongxi110)inducedbyparthenogeneticinducing1ineTheresultsshowedthat，therewasnosegregationdistor

8、tionamong30markersof“DengllaiNo9”and24markersof(Nongxi531K36)While3linkedmarkers，locatingthesecondchromosome，showedthesegregationdistortion(P005)andderivedtowardfemaleparent(Nongxi531)among39markersofDHpopulation372lines

9、ofDHpopulationderivedfrom“NongDanNo5”(Nongxi531Nongxi110)wereevaluatedforearlength，earperimeterearweight，cobperimetercobwei曲t，100，lernelweie#t，rowNo，kernel／rowrareearlength，planthei曲t，stemperimeteruJP，LUN，numberoflateral