光化學微球傳感器的材料及應(yīng)用研究.pdf

1、天津大學碩士學位論文光化學微球傳感器的材料及應(yīng)用研究姓名：傅英松申請學位級別：碩士專業(yè)：材料學指導教師：許鑫華20021201摘要ABSTRACTBiolo西calstudiesatthesinglecelIleveIhaveattractedtheattentionofresearchersfrommanydisciplinesRealtime，online，inVIVO，加situmeasurementoftheconcentrat

2、ionanddistributionofdifferentspecieswitllincellsandcellularcomponentswillprovideusefn1informationfortheresearchofmedicalandbiologyInthispaperanewtypenanosensorsnamedPEBBLEs(ProbesEncapsulatedbyBiologicallyLocalizedEmbedd

3、in91areintroduced，whichisa“flbrelessopticalnanosensors”Itjsalsocalledpolymernanospherebasedf】uorescentSeDSOrSbecausefluorescentjndicatorisappliedtolabelnanosphere’rlaisnewmethodintegratesnanotechnologybiomedicaltechnolog

4、yandfluorescenceanalyticaltechnologywhichovercomesthedefectoftraditionalfluorescentanalyticalmethodsSoPEBBLEsareidealtoolstoquantitativelyanalyzemanyspecieswithincellsInthispapeLnanosphoresarepreparedbyinversephrasemicro

5、emulsionpolymerization(AMandNVPasmonomer)first，thenthehydrolysateofTetraethylorthosilicate(TEOS)asmatrix，fluorescentindicator(Fura2)asnucleus，fluorescencenanosphereswithSi02shellarefabricatedbysol—gelmethodatroonqtempera

6、tureconditionThefabricationnanospheresareusedtomeasuretheconcentrationofCa”invitroThemalnconclusionsareasfollows：InversephrasemicroemusionpolymerizationmethodworkswithAlvlasmonomerTheratiobetweenoilandwaterisgreaterthan1

7、5betweenAMandN，Nmethylenebis(aerylamide)isequalto9，thecontentofemulsificationisequalt03gramandthecontentofinitiatorisequalto54mL’VheaveragediameterDofnanospheresisabout70rimaccordingtothestatisticalcalculalionInversephms

8、emiercemusionpolymerizationmethodworkswithNVPasmonomerN，Nmethylen曲is(acrylamide)isusedascrosslinkinginordertogethighyieldthetemperatureisabout70“C：thetimeofreactionis2～3址thecontentofinitiatorisabout018percentofamountofmo

9、nomerandtheratiobetweenfNIhhS208madTEMEDis2：theratiobetweenemulsificationAOTandaidedemulsificationMOAjs2Especiallyifxyleneisusedassolutiont11ediameterofnanosphereis100nmW1lensolgelmethodisadoptedTEOSjsusedastheprecIMsora

10、ndtheratiobetweenH20andTEOSisgreaterthan50atthesametime。withammoniausedascatalystPEGasstabilizerethanolascosolvent，nanospherescanbeprepared，thediameteris300nmWhenfura2isusedasnucleus(theflnaJconcentrationoffura2isoverl04

11、)，fluorescencenanosphereswithSi02shellcanbeusedtomeasuretheconcentrationofCa2Thefollowingjobistoalterthetypeoffluorescentindicatorbasedontheestablishedtechniqueandtoap口1Ynanosensorstobiology(suchascells)iflordertoanalyze

12、spectesquantitativelyAtthesametime，nanosensorsaremodifiedbiologicallyandbiologicalfluorescencenanospheresareobtainedwithrecognitionfunctioninparticularsothebimolecularinteractioncanbeinvestigatedKeywords：ProbesEncapsulat