抗菌肽Trpi免疫調(diào)節(jié)機(jī)理的研究.pdf

1、天津商業(yè)大學(xué)碩士學(xué)位論文抗菌肽Trpi免疫調(diào)節(jié)機(jī)理的研究姓名：黃栩林申請學(xué)位級(jí)別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：龐廣昌20090501AbstractPolypeptidewhichissinglewasseparatedfromtrypticcaseinhydrolysatesbyisoelectricpointsedimentation，ethanolsedimentation，ultrafiltrationandtworoundso

2、fSephadexG一25chromatography，anditslethalityrateforStaphylococcusaureusis713％ThereforeitisassuredthatthesepolypeptidesarethereportorialantibacterialpeptideTrpiTheprophaseexperimentssuggestedthatTrpimaybecombinetotherecept

3、ors，especiallyTLR4，ontheantigenpresentingcells(APCs)intheintestinalmucosal，andthenitcouldactiveTLR4signalingpathwayFinally，theactivednucleartranscriptionfactortranscriptedcytokines’mRNAandproducedthesecytokines，IL113，IL6

4、Afterwards，IL6wouldenhancetheIL4productionbyitsreceptorIL6R，whichmakesTh0polarizetoTh2Ingeneral，thecytokinenetworkinhostisdynamicbalanceWhenthepresenceofIL113，IL一6，theywouldexcitetheirsignalingpathwaythroughcytokinerecep

5、toronothercells，andthenmotivateanti—inflammationcytokines，suchasIL一10ImportantlyIL6andIL10couldincreaseBcellssurvavl，promoteBcellsdifferentiation，andassistBcellstoproduceantibodyInotherwords，Trpicouldmodulatethecytokinen

6、etworkthroughintestinalmucosalimmunitysystem，then，regulatehost’Sinnateimmunity，andthecytokineswouldinitiateadaptiveimmunityInordertoinvestigatefurtherTrpi’Simmunomodulatorpropertyonadaptiveimmunity，weculturedDCsderivedmi

7、ce’Sbonemarrow，andstudiedinitiallytheprocesswhichTrpiactedonDCsItwasthatalagernumberofstudieshavebeenindicatedthatdendriticcellsplayacrucialroleininnateimmunityandadaptiveimmunityTheresultsrevealedthatTrpistimulatedCD86e

8、xpressionintensively，andCD80，IL一12p40，IP10expressionseemtodependonTrpi’SconcentrationAccordingtocytokinesecretioninvivo，itimpliesthatTrpicouldactdirectlyonimmatureDCstoinducetheirfunctionalmatureandenablesthemtoelicitaro