幾種物理因素對(duì)紅豆杉懸浮培養(yǎng)細(xì)胞的影響.pdf

1、華中科技大學(xué)碩士學(xué)位論文幾種物理因素對(duì)紅豆杉懸浮培養(yǎng)細(xì)胞的影響姓名：董妍玲申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化工指導(dǎo)教師：梅興國(guó)2002.1.22華中科技大學(xué)碩士學(xué)位論文AbstractInordertoenhancethecellgrowthandtaxolproduction，effectsofseveralphysicalfactorsonthesuspensionculturesofTaxuschinesiscellswerestud

2、iedandthemechanismoftheseeffectsweresimplyexploredEffectsofpulseelectricityof25～175V／cmplus1～7hrtreatmentoncellsonday7and20werestudiedItwasfoundthattaxolyieldswere2～9foldsofthatofthecontrolanditsreleasepercentagewasincre

3、asedto4050％from10％Simultaneously，thecytoactivitymembranerelativepermeabilityandactivityofsuperoxidedesmutase(SOD)weregreatlyincreasedinshorttime(19hr)aftertreatmentWeakalternatingcurrentof18～126uAstimulationtocellsonday2

4、1couldimprovetaxolyieldsto57mg／LO55hrhightemperaturetreatmentof42℃showedthatshorttimetreatmentofcellsonday7couldeffectivelyincreaseenzymeactivitiesofSOD，Peroxidase(POD)andPhenylalanineLyase(PAL)Howeverabout35hrtreatmentl

5、oweredcytoactivityandincreasedcelldeathrateAsfortaxolproductivity，effectsof42℃treatmentonday14werebetterthanMatonday7，withthehighestyieldsof421mg／LMutualeffectsof42℃andseveralchemicalfactorssuchasmethyljasmonate(MJ)，fung

6、uselicitor(F5)andsalicylicacid(SA)showedthefourhadagoodsynergisticaction，whichpromotedthetaxolyieldsabout27timesTreatmentofmicrowaveirradiationoncellsshowedthatl60Wmicrowavepartlyinhibitedthecellgrowthbutitslightlyincrea

7、sedtaxolyields320Wand480WmicrowaveirradiationcoulddistinctlyincreasetheenzymeactivitiesofPODandPALBy640W’30smicrowavetreatment，taxolyieldWaSupto5medL，4timesofthecontrol。andthetaxolreleaserateincreasedfroml0％to20～30％Keywo