吉林省蝙蝠科三種蝙蝠的核型研究.pdf

1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文吉林省蝙蝠科三種蝙蝠的核型研究姓名：高志英申請學(xué)位級別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：姜云壘20080601StudyonKaryotypesofThreeSpeciesofVespertilionidaeBatsfromJilinAbstractChromosomepreparationsweremadefromculturecellsfrombloodofheartThechromosomeslideswer

2、emadebythestandardairdryingmethodnekaryotypesGbandsandCbandsofthreespeciesofVespertilionidaeBats(Myotisfolrmosuswalasei，MyoOsikonnikovi，MiniopterusschreibersOfromJilinwereanalyzed111eGbandtechniquereportedbySeabfight(197

3、1)andCbandtechniquereportedbySumner(1972)wereappliedTheresultswereasfollowed：①Ofthe3speciesbats，thekaryotypesofMyotisformosuswalaseiandMyotisikonnikoviwerereportedforthefirsttimeinChina11圯diploidchromosomesnumberandthefu

4、ndamentalnumberofautosomalarms(EN)ofMyoUsformos“swalaseiandMyotisikonnikoviareidentical，theyare2n244，NE250TheirkaryotypespossessallthethreecharacteristicsofstandardkaryotypedataofMyotis：First，thediploidchromosomesnumbero

5、fallspeciesofMyotisis2n=44；Secondmostspeciesofallpossess3pairsofbigmetaeentricorsubmetacentricchromosomesandlpairofmediam—sizedmetacentricorsubmetacentricones；ThirdtheXchromosomeofallspeciesofMyoasiSmediumsizedmetacentri

6、corsubmetacentric，TheYchromosomeiStelocentricThediploidchromosomenumberandthefundamentalnumberofautosomalarmsofMiniopterusschreibersiiscoincidencewiththatreportedbyGuXiaoming，thatis2n=46，NF=50，anditsFNisidenticalwiththat

7、ofMyotis②TlleGbandofthreespeciesbatshadnotbeenpreviouslyreportedinchinaTheGbandsofMyotisfo]7“IOSUSwalaseiandMyonsikonn／koviweresimilarwiththoseofMyotisreportedbyBickham，andwehaveprovedagainthatMyotisjbatshaveextremelysta

8、bleandconservativekaryotypeinthisgenusneGbandofMiniopterusschreibersiwaspartlyhomologouswiththoseoftwospicesofMyotis，theNo1，3，4，5，19，20，21andXchromosomesofMyotJsarehomologouswiththeNo1，2，4，3，20，21，22andXonesofMiniopterus

9、⑨mC—bandofthreespeciesbatshadnotbeenpreviouslyreportedinchinaAllthethreespeciesofbatspossessedccntromericheterochromatins，butinterstitialheterochromatindistributedontheNo1，2，3，4，9，ll，14chromosomeandthelongannoftheNo13chr

10、omosomeandterminalheterochromatindistributedontheNo4and14chromosomeofMyoasformosuswalasei④TheevolutionaryrelationshipbetweenMyoasandMiniopteruswasanalyzedaccordingtotheirsimilaritiesanddifferencesinkaryotype，G—bandandCba