鹵蟲無節(jié)幼體急性毒性實(shí)驗(yàn)方法的標(biāo)準(zhǔn)化研究.pdf

1、天津科技大學(xué)碩士學(xué)位論文鹵蟲無節(jié)幼體急性毒性實(shí)驗(yàn)方法的標(biāo)準(zhǔn)化研究姓名：陳婕申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：化學(xué)工藝指導(dǎo)教師：張波200902ABSTRACT彳廠fP坍婦naupliiaremoreaIIdmoreusedintheacutetoxicitytestsaSexaminedbiolOgyBecauseofthedifferentexperimentalmethods，theresultswereimpreciseandincompa

2、rableW色Ch00se5straillsof彳脅期缸whichwereGSL，SGAB，BH卸dBYt0reseaIIchtheacutetoxicilytestS’methodof彳朋冊(cè)l婦n踟pliiTheLc50H曠ofdi婦Ferentstrainsof彳，陀m婦naupliiwereGSL2879mg／乙SG611mg／LAB1096m∥乙BH1264mg／LBY1644mg／Lintum么胞m婦cystsofGSLdon

3、otonlyhave900dqualityhi曲production，butalsoareaccessibleandwidelyusedThestablecreaturetraitistheInostimport鋤tfactor0f彳，f繃婦naupliiwhicharehatchedf幻mthesancystsT1lerefore，GSL彳rf冊(cè)z缸werechos明弱experimentalmaterials0fthefollowu

4、pexperimentsWeaScenajnedthest鋤dardmethodwmchw雒obtainingthesynchronoushatched爿r紀(jì)m婦n加pliiinthesameage，basedontheresearchof4r紀(jì)晰砌cysts’HR趾dthemoltingtimeof彳，f巴，咒如naupli衛(wèi)1etoleranccsofiIlstarI獅dinstarII么廠fgm腸t0H礦weredi由陀rent，

5、thelc50were2448mg／LaIld458mg／L；neL，C50H礦ofIo，I5，Ill，I16were2279mg幾1926mg／L，1756mg兒557mg／Lintu】fnItme趾sthatthetoler觚cesof4m冊(cè)誼iIldi虢rentinstars觚dagesweresignificaIltlydi伍erentAndtheIc50—Hgzofjunior鋤d彳，fe所婦adultwere6135mg／L

6、粕d449Ing／LrespeCtivelymordertono冊(cè)alizetlleexperimentmethods，we∞lected彳胞m缸naupliiwhichweresynchronousandjusthatched(10)t0proceedtheacutetOxidtytestsOnthebaseofthestandardmemods，wedeteminedUC50ofdi任brentkindsoftoxinneLc50o

7、fdi能rentⅪndsofheavymetalionswereCd抖66575m嘰Hg計(jì)2448m嘰Pb230934mg兒C’r37882mg幾Cu26687mg／Lint啪111esequenccOftOxicitywasH礦cu“口Pb2Cd2；111eLC50NH4w雒664103mg／LDi鼢ento瑪anictoxinhavedi￡眙rente￡fects0nthedeathrateof彳，f繃砌naupliiTheLC卯w

8、ereCH201591曠mg化SDS2093mg兒dieseloil65811mg化phoxim23454mg／Lintum觚ordingtostudyingon4廠l咖缸cysts’HR鋤dthemol缸gtinleof彳療繃婦naupli，wefoundthest觚dardmethodwllichw嬲0btainingthesynchronoushatchedinstar14腴聊缸forthefirsttiIlleTheimport

9、觚ceof丘xingthestI面n，instar她dmeaget0theacutetoxidtytestsw髂madedeallyWbsetupthest卸dardmethod0ftlleacutetoxicitytestsofA啊巴，打缸naupliiiIlitially，姐dth∞proceededtheacutetoxicitytestsofdi岱：renttoxinsystematicallyKeywords：biolo酉ca