胎盤凋亡基因bcl-2、Fasl的表達(dá)與母血、羊水中EGF、ET的關(guān)系研究.pdf

1、遵義醫(yī)學(xué)院碩士學(xué)位論文胎盤凋亡基因bcl2、FasL的表達(dá)與母血、羊水中EGF、ET的關(guān)系研究姓名：張有成申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：王豫黔2003.6.10畢業(yè)論文：胎盤凋亡基因bcl一2、Fasl的表達(dá)與母血、羊水中EGF、ET的關(guān)系研究4Arelationshipbetweentheexpressionoftwoapoptosisgenesinplacental，bel2andFaslandthechangeofEG

2、FandETinmaternalbloodandamnioticfluid[Abstract]objectiveToinvestigatetheexpressionofbcl一2andFaslproteinsinplacentaltrophoblasticcellsduringthecourseof1aborandtheirrelationshipwithEpidermolGrowthFacters(EGFlandEndothelins

3、(ET)inmatemalbloodandamnionoticfluidMethodsFiftythreewomenontermpregnancyweredividedintotwogroupsOnegroupOnthetimeoflaboronsetweretwenty—eightwomenbyspontaneousvaginaldeliverAnothergroupnotonthetimeof1aboronsetweretwenty

4、fivewomenbyselectivecesareansectionwithoutanybirthpainExpressionofbcl2andFas】proteinsinplacentaltrophoblasticcellsweredetectedbyimmunohistochemicalassayusingmonoclonalantibodyEGFandETinmatemaIbloodandamnionoticfluidweted

5、etectedbyradioimmuneassayResults(1)bcl2andFaslproteinswereexpressedintermplacentaltrophoblasticcellsf2)Expressionsofbcl一2andFaslproteinsingrouplaboronsetwassignificantlylowerthanthegroupnotlaboronsetTherewasastatisticall

6、ydifferenceintheaveragegrayandtheratioofpositivecellsbetweenth，etwogroups(pO05)(3)TheconcentrationsofEGFandETinmaternalbloodandamnionoticfluidoftwogroupswerehigherthannormal。Buttheconcentrationsinlaboronsetgroupwerehighe